ArticleMolecular biology of the cell2005
Early spindle assembly in Drosophila embryos: role of a force balance involving cytoskeletal dynamics and nuclear mechanics.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 64 citations in OpenAlex.
- Mechanical states of a motor protein in the spindle.Current biology : CB · 2026Article
- Kinesin-1 patterns Par-1 and Rho signaling at the cortex of syncytial embryos of Drosophila.The Journal of cell biology · 2024Article
- Attractive internuclear force drives the collective behavior of nuclear arrays in Drosophila embryos.PLoS computational biology · 2021Article
- Excess dNTPs Trigger Oscillatory Surface Flow in the Early Drosophila Embryo.Biophysical journal · 2020Article
- Mechanisms of chromosome biorientation and bipolar spindle assembly analyzed by computational modeling.eLife · 2020Article
- Self-Similar Dynamics of Nuclear Packing in the Early Drosophila Embryo.Biophysical journal · 2019Article
- Theory of Cytoskeletal Reorganization during Cross-Linker-Mediated Mitotic Spindle Assembly.Biophysical journal · 2019Article
- Precocious cleavage furrows simultaneously move and ingress when kinetochore microtubules are depolymerized in Mesostoma ehrenbergii spermatocytes.Protoplasma · 2018Article
- Computer simulations reveal mechanisms that organize nuclear dynein forces to separate centrosomes.Molecular biology of the cell · 2017Article
- Physical determinants of bipolar mitotic spindle assembly and stability in fission yeast.Science advances · 2017Article
- Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast.Molecular biology of the cell · 2016Article
- Universally Conserved Relationships between Nuclear Shape and Cytoplasmic Mechanical Properties in Human Stem Cells.Scientific reports · 2016Article
- Fluctuation Analysis of Centrosomes Reveals a Cortical Function of Kinesin-1.Biophysical journal · 2015Article
- The Msd1-Wdr8-Pkl1 complex anchors microtubule minus ends to fission yeast spindle pole bodies.The Journal of cell biology · 2015Article
- Review
- Pericentric chromatin loops function as a nonlinear spring in mitotic force balance.The Journal of cell biology · 2013Article
- Transient defects of mitotic spindle geometry and chromosome segregation errors.Cell division · 2012Article
- Actin behavior in bulk cytoplasm is cell cycle regulated in early vertebrate embryos.Journal of cell science · 2011Article
- Actomyosin-dependent cortical dynamics contributes to the prophase force-balance in the early Drosophila embryo.PloS one · 2011Article
- A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophila embryos.Journal of cell science · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Mitotic spindle morphogenesis depends upon the action of microtubules (MTs), motors and the cell cortex. Previously, we proposed that cortical- and MT-based motors acting alone can coordinate early spindle assembly in Drosophila embryos. Here, we tested this model using microscopy of living embryos to analyze spindle pole separation, cortical reorganization, and nuclear dynamics in interphase-prophase of cycles 11-13. We observe that actin caps remain flat as they expand and that furrows do not ingress. As centrosomes separate, they follow a linear trajectory, maintaining a constant pole-to-furrow distance while the nucleus progressively deforms along the elongating pole-pole axis. These observations are incorporated into a model in which outward forces generated by zones of active cortical dynein are balanced by inward forces produced by nuclear elasticity and during cycle 13, by Ncd, which localizes to interpolar MTs. Thus, the force-balance driving early spindle morphogenesis depends upon MT-based motors acting in concert with the cortex and nucleus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.