ArticleMolecular biology of the cell2005
Measuring the stoichiometry and physical interactions between components elucidates the architecture of the vertebrate kinetochore.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 70 citations in OpenAlex.
- Aurora A phosphorylates Ndel1 to reduce the levels of Mad1 and NuMA at spindle poles.Molecular biology of the cell · 2023Article
- Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts.Molecular biology of the cell · 2021Article
- CLASP2 binding to curved microtubule tips promotes flux and stabilizes kinetochore attachments.The Journal of cell biology · 2020Article
- Enrichment of Aurora B kinase at the inner kinetochore controls outer kinetochore assembly.The Journal of cell biology · 2019Article
- Chmp4c is required for stable kinetochore-microtubule attachments.Chromosoma · 2018Article
- Distinct Roles of the Chromosomal Passenger Complex in the Detection of and Response to Errors in Kinetochore-Microtubule Attachment.Developmental cell · 2017Article
- Mechanism of Ska Recruitment by Ndc80 Complexes to Kinetochores.Developmental cell · 2017Article
- Bub1 targeting to centromeres is sufficient for Sgo1 recruitment in the absence of kinetochores.Chromosoma · 2017Article
- Review
- Minimal model for collective kinetochore-microtubule dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Kinetochore function is controlled by a phospho-dependent coexpansion of inner and outer components.The Journal of cell biology · 2015Article
- The distinct functions of CENP-C and CENP-T/W in centromere propagation and function in Xenopus egg extracts.Nucleus (Austin, Tex.) · 2015Article
- CENP-I and Aurora B act as a molecular switch that ties RZZ/Mad1 recruitment to kinetochore attachment status.The Journal of cell biology · 2014Article
- New insights into the mechanism for chromosome alignment in metaphase.International review of cell and molecular biology · 2013Review
- Counting protein molecules using quantitative fluorescence microscopy.Trends in biochemical sciences · 2012Review
- Sos7, an essential component of the conserved Schizosaccharomyces pombe Ndc80-MIND-Spc7 complex, identifies a new family of fungal kinetochore proteins.Molecular and cellular biology · 2012Article
- Cdt1 throws kinetochore-microtubule attachments for a loop.Nature cell biology · 2012Article
- Multiple domains of human CLASP contribute to microtubule dynamics and organization in vitro and in Xenopus egg extracts.Cytoskeleton (Hoboken, N.J.) · 2012Article
- Point centromeres contain more than a single centromere-specific Cse4 (CENP-A) nucleosome.The Journal of cell biology · 2011Article
- The N-terminal coiled-coil of Ndel1 is a regulated scaffold that recruits LIS1 to dynein.The Journal of cell biology · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Vertebrate kinetochores contain over 50 different proteins organized into three distinct regions: the inner plate, outer plate, and fibrous corona. The present study characterizes numerous precursors of kinetochore assembly in a system free of centromeric chromatin, Xenopus extracts. Hydrodynamic analysis suggests there are a minimum of two monomeric proteins and six pre-assembled complexes that accumulate on centromeres to form the kinetochore. The inner and outer kinetochore assemble from at least two distinct kinetochore complexes containing the proteins Mis12, Zwint, and Ndc80, all of which interact by immunoprecipitation. There is also a network of interactions between the fibrous corona proteins that is dissociated by microtubules. We quantify the number of molecules of specific proteins assembled into a single kinetochore. There are between 800 and 1200 molecules of the measured inner and outer kinetochore proteins, demonstrating that the components in these regions are in similar stoichiometry. In contrast, the measured fibrous corona proteins are present at 250-300 molecules per kinetochore. Zwint, but not Mis12, requires the Ndc80 complex for assembly into the kinetochore. Further, Ndc80 requires Zwint for assembly, indicating a co-dependency for these two proteins. Our data provide a model for the structural architecture and assembly pathway of the vertebrate kinetochore.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.