ArticleJournal of bacteriology2005
Identification of a 123-kilodalton protein (Gli123) involved in machinery for gliding motility of Mycoplasma mobile.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 68 citations in OpenAlex.
- Nano-Scale Video Imaging of Motility Machinery by High-Speed Atomic Force Microscopy.Biomolecules · 2025Review
- Gliding direction ofBiophysics and physicobiology · 2025Article
- Internal structure ofBiophysics and physicobiology · 2024Article
- Structure and Function of Gli123 Involved in Mycoplasma mobile Gliding.Journal of bacteriology · 2023Article
- Detection of Steps and Rotation in the Gliding Motility of Mycoplasma mobile.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Chained Structure of Dimeric FmBio · 2021Article
- Movements of Mycoplasma mobile Gliding Machinery Detected by High-Speed Atomic Force Microscopy.mBio · 2021Article
- Identification and sequence analyses of the gliding machinery proteins from Mycoplasma mobile.Scientific reports · 2020Article
- ExploringFrontiers in microbiology · 2020Review
- Article
- Identification of novel protein domain for sialyloligosaccharide binding essential to Mycoplasma mobile gliding.FEMS microbiology letters · 2019Article
- Linear motor driven-rotary motion of a membrane-permeabilized ghost in Mycoplasma mobile.Scientific reports · 2018Article
- Detailed Analyses of Stall Force Generation in Mycoplasma mobile Gliding.Biophysical journal · 2018Article
- Novel mechanisms power bacterial gliding motility.Molecular microbiology · 2016Review
- Article
- Gliding Direction of Mycoplasma mobile.Journal of bacteriology · 2016Article
- Gliding Motility of Mycoplasma mobile on Uniform Oligosaccharides.Journal of bacteriology · 2015Article
- Unitary step of gliding machinery in Mycoplasma mobile.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Localization of P42 and F(1)-ATPase α-subunit homolog of the gliding machinery in Mycoplasma mobile revealed by newly developed gene manipulation and fluorescent protein tagging.Journal of bacteriology · 2014Article
- Protein kinase/phosphatase function correlates with gliding motility in Mycoplasma pneumoniae.Journal of bacteriology · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycoplasma mobile glides on a glass surface in the direction of its tapered end by an unknown mechanism. Two large proteins, Gli349 and Gli521, were recently reported to be involved in glass binding and force generation/transmission, respectively, in M. mobile gliding. These proteins are coded tandemly with two other open reading frames (ORFs) in the order p123-gli349-gli521-p42 on the genome. In the present study, reverse transcriptase PCR analysis suggested that these four ORFs are transcribed cistronically. To characterize the p123 gene coding a 123-kDa protein (Gli123) of 1,128 amino acids, we raised polyclonal antibody against the Gli123 protein. Immunoblotting for Gli123 revealed that Gli123 was missing in a mutant strain, m12, which was previously isolated and characterized by a deficiency in glass binding. Sequencing analysis showed a nonsense mutation at the 523rd amino acid of the protein in the m12 mutant. Immunofluorescence microscopy with the polyclonal antibody showed that Gli123 is localized at the head-like protrusion's base, the cell neck, which is specialized for gliding, as observed for Gli349 and Gli521. Localization of the gliding proteins, Gli349 and Gli521, was disturbed in the m12 mutant, suggesting that Gli123 is essential for the positioning of gliding proteins in the cell neck.
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