ArticleJournal of bacteriology2005
Methanol-dependent gene expression demonstrates that methyl-coenzyme M reductase is essential in Methanosarcina acetivorans C2A and allows isolation of mutants with defects in regulation of the methanol utilization pathway.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Seven critical challenges in synthetic one-carbon assimilation and their potential solutions.FEMS microbiology reviews · 2025Review
- A promoter-RBS library for fine-tuning gene expression inApplied and environmental microbiology · 2024Article
- Methanogens: biochemical background and biotechnological applications.AMB Express · 2018Review
- Article
- Cas9-mediated genome editing in the methanogenic archaeonProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Assessing methanotrophy and carbon fixation for biofuel production by Methanosarcina acetivorans.Microbial cell factories · 2016Article
- Rerouting Cellular Electron Flux To Increase the Rate of Biological Methane Production.Applied and environmental microbiology · 2015Article
- Development of β -lactamase as a tool for monitoring conditional gene expression by a tetracycline-riboswitch in Methanosarcina acetivorans.Archaea (Vancouver, B.C.) · 2014Article
- MreA functions in the global regulation of methanogenic pathways in Methanosarcina acetivorans.mBio · 2012Article
- Genetic manipulation of Methanosarcina spp.Frontiers in microbiology · 2012Article
- A novel inducible protein production system and neomycin resistance as selection marker for Methanosarcina mazei.Archaea (Vancouver, B.C.) · 2012Article
- Metabolic reconstruction of the archaeon methanogen Methanosarcina Acetivorans.BMC systems biology · 2011Article
- Article
- Differences in hydrogenase gene expression between Methanosarcina acetivorans and Methanosarcina barkeri.Journal of bacteriology · 2009Article
- New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species.Archaea (Vancouver, B.C.) · 2008Article
- Genetic analysis of the methanol- and methylamine-specific methyltransferase 2 genes of Methanosarcina acetivorans C2A.Journal of bacteriology · 2008Article
- Quantitative proteomic and microarray analysis of the archaeon Methanosarcina acetivorans grown with acetate versus methanol.Journal of proteome research · 2007Article
- Differential regulation of the three methanol methyltransferase isozymes in Methanosarcina acetivorans C2A.Journal of bacteriology · 2006Article
- The evolutionary history of Cys-tRNACys formation.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methanosarcina acetivorans C2A is able to convert several substrates to methane via at least four distinct methanogenic pathways. A common step in each of these pathways is the reduction of methyl-coenzyme M (CoM) to methane catalyzed by methyl-CoM reductase (MCR). Because this enzyme is used in each of the known pathways, the mcrBDCGA operon, which encodes MCR, is expected to be essential. To validate this prediction, a system for conditional gene inactivation was developed. A heterologous copy of the mcrBDCGA operon was placed under the control of the highly regulated mtaC1 promoter, which directs the expression of genes involved in methanol utilization, and recombined onto the M. acetivorans chromosome. This allowed for disruption of the endogenous mcr operon in the presence of methanol. Because the PmtaC1 promoter is transcribed only during growth on methanol, mcrBDCGA was rendered methanol dependent and the strain was unable to grow in trimethylamine media, strongly suggesting that mcrBDCGA is essential. Upon prolonged incubation, suppressed mutants which expressed mcrBDCGA constitutively could be selected. Expression analysis of PmtaC1::uidA gene fusions in several isolated suppressed mutants suggests that they carry trans-active mutations leading to deregulation of all genes under control of this promoter. Subsequently, proteome analysis of one such suppressed mutant revealed that all known proteins derived from mtaC1 promoter-dependent expression were constitutively expressed in this mutant. This genetic system can therefore be employed for the testing of essential genes and for the identification of genes under a common regulatory mechanism by making regulatory mutations phenotypically selectable.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.