ArticleDevelopment (Cambridge, England)2005
Dynein and the actin cytoskeleton control kinesin-driven cytoplasmic streaming in Drosophila oocytes.
Article in Development (Cambridge, England), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
76 citing papers in PubMed, 148 citations in OpenAlex.
- The Influence of Drosophila Spire and Myosin V During Mid-Oogenesis Is Independent of Their Direct Interaction.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Article
- Review
- Visualization and Analyses of Cytoplasmic Streaming in C. elegans Zygotes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Kinesin KIF3A regulates meiotic progression and spindle assembly in oocyte meiosis.Cellular and molecular life sciences : CMLS · 2024Article
- Unraveling the interplay of kinesin-1, tau, and microtubules in neurodegeneration associated with Alzheimer's disease.Frontiers in cellular neuroscience · 2024Review
- Article
- Fly embryo nuclei riding on two-fluid flow.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Distinct Wolbachia localization patterns in oocytes of diverse host species reveal multiple strategies of maternal transmission.Genetics · 2023Article
- Go with the flow - bulk transport by molecular motors.Journal of cell science · 2023Review
- Size- and position-dependent cytoplasm viscoelasticity through hydrodynamic interactions with the cell surface.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Competing instabilities reveal how to rationally design and control active crosslinked gels.Nature communications · 2022Article
- A cytoskeletal vortex drives phage nucleus rotation during jumbo phage replication in E. coli.Cell reports · 2022Article
- Polarity Events in theFrontiers in cell and developmental biology · 2022Review
- Article
- Spontaneous circulation of active microtubules confined by optical traps.Journal of biological physics · 2021Article
- Receptor-mediated yolk uptake is required for oskar mRNA localization and cortical anchorage of germ plasm components in the Drosophila oocyte.PLoS biology · 2021Article
- Swirling Instability of the Microtubule Cytoskeleton.Physical review letters · 2021Article
- Patterning the Drosophila embryo: A paradigm for RNA-based developmental genetic regulation.Wiley interdisciplinary reviews. RNA · 2020Review
16 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Mass movements of cytoplasm, known as cytoplasmic streaming, occur in some large eukaryotic cells. In Drosophila oocytes there are two forms of microtubule-based streaming. Slow, poorly ordered streaming occurs during stages 8-10A, while pattern formation determinants such as oskar mRNA are being localized and anchored at specific sites on the cortex. Then fast well-ordered streaming begins during stage 10B, just before nurse cell cytoplasm is dumped into the oocyte. We report that the plus-end-directed microtubule motor kinesin-1 is required for all streaming and is constitutively capable of driving fast streaming. Khc mutations that reduce the velocity of kinesin-1 transport in vitro blocked streaming yet still supported posterior localization of oskar mRNA, suggesting that streaming is not essential for the oskar localization mechanism. Inhibitory antibodies indicated that the minus-end-directed motor dynein is required to prevent premature fast streaming, suggesting that slow streaming is the product of a novel dynein-kinesin competition. As F-actin and some associated proteins are also required to prevent premature fast streaming, our observations support a model in which the actin cytoskeleton triggers the shift from slow to fast streaming by inhibiting dynein. This allows a cooperative self-amplifying loop of plus-end-directed organelle motion and parallel microtubule orientation that drives vigorous streaming currents and thorough mixing of oocyte and nurse-cell cytoplasm.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.