ArticleNucleic acids research2005
Unfolding of DNA quadruplexes induced by HIV-1 nucleocapsid protein.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- A high-throughput approach to evaluating NCp7 RNA binding activity for HIV-1 drug discovery.SLAS discovery : advancing life sciences R & D · 2025Article
- The human cellular protein NoL12 is a specific partner of the HIV-1 nucleocapsid protein NCp7.Journal of virology · 2023Article
- Recent advances in applying G-quadruplex for SARS-CoV-2 targeting and diagnosis: A review.International journal of biological macromolecules · 2022Review
- HIV-1 Nucleocapsid Protein Unfolds Stable RNA G-Quadruplexes in the Viral Genome and Is Inhibited by G-Quadruplex Ligands.ACS infectious diseases · 2019Article
- CNBP controls transcription by unfolding DNA G-quadruplex structures.Nucleic acids research · 2019Article
- G-Quadruplex Forming Oligonucleotides as Anti-HIV Agents.Molecules (Basel, Switzerland) · 2015Review
- G-quadruplexes in viruses: function and potential therapeutic applications.Nucleic acids research · 2014Review
- Structural transformation induced by locked nucleic acid or 2'-O-methyl nucleic acid site-specific modifications on thrombin binding aptamer.Chemistry Central journal · 2014Article
- Quadruplex-forming sequences occupy discrete regions inside plant LTR retrotransposons.Nucleic acids research · 2014Article
- HIV-1 nucleocapsid proteins as molecular chaperones for tetramolecular antiparallel G-quadruplex formation.Journal of the American Chemical Society · 2013Article
- Role of conserved cis-regulatory elements in the post-transcriptional regulation of the human MECP2 gene involved in autism.Human genomics · 2013Article
- QGRS-H Predictor: a web server for predicting homologous quadruplex forming G-rich sequence motifs in nucleotide sequences.Nucleic acids research · 2012Article
- G4 resolvase 1 tightly binds and unwinds unimolecular G4-DNA.Nucleic acids research · 2011Article
- HIV-1 nucleocapsid protein increases strand transfer recombination by promoting dimeric G-quartet formation.The Journal of biological chemistry · 2011Article
- C-terminal domain modulates the nucleic acid chaperone activity of human T-cell leukemia virus type 1 nucleocapsid protein via an electrostatic mechanism.The Journal of biological chemistry · 2010Article
- Disorder-to-order conformational transitions in protein structure and its relationship to disease.Molecular and cellular biochemistry · 2009Review
- Applications of isothermal titration calorimetry in biophysical studies of G-quadruplexes.International journal of molecular sciences · 2009Review
- Protein hnRNP A1 and its derivative Up1 unfold quadruplex DNA in the human KRAS promoter: implications for transcription.Nucleic acids research · 2009Article
- Fidelity of plus-strand priming requires the nucleic acid chaperone activity of HIV-1 nucleocapsid protein.Nucleic acids research · 2009Article
- Effect of Mg(2+) and Na(+) on the nucleic acid chaperone activity of HIV-1 nucleocapsid protein: implications for reverse transcription.Journal of molecular biology · 2009Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The human immunodeficiency virus type 1 nucleocapsid protein (NC) is a nucleic acid chaperone that catalyzes the rearrangement of nucleic acids into their thermodynamically most stable structures. In the present study, a combination of optical and thermodynamic techniques were used to characterize the influence of NC on the secondary structure, thermal stability and energetics of monomolecular DNA quadruplexes formed by the sequence d(GGTTGGTGTGGTTGG) in the presence of K+ or Sr2+. Circular dichroism studies demonstrate that NC effectively unfolds the quadruplexes. Studies carried out with NC variants suggest that destabilization is mediated by the zinc fingers of NC. Calorimetric studies reveal that NC destabilization is enthalpic in origin, probably owing to unstacking of the G-quartets upon protein binding. In contrast, parallel studies performed on a related DNA duplex reveal that under conditions where NC readily destabilizes and unfolds the quadruplexes, its effect on the DNA duplex is much less pronounced. The differences in NC's ability to destabilize quadruplex versus duplex is in accordance with the higher DeltaG of melting for the latter, and with the inverse correlation between nucleic acid stability and the destabilizing activity of NC.
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