ArticleMammalian genome : official journal of the International Mammalian Genome Society2005
Efficient and fast targeted production of murine models based on ENU mutagenesis.
Article in Mammalian genome : official journal of the International Mammalian Genome Society, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- PHEXGenes · 2022Article
- Improvement of ENU Mutagenesis Efficiency Using Serial Injection and Mismatch Repair Deficiency Mice.PloS one · 2016Article
- A new Otogelin ENU mouse model for autosomal-recessive nonsyndromic moderate hearing impairment.SpringerPlus · 2015Article
- Suppressor of cytokine signaling 4 (SOCS4) protects against severe cytokine storm and enhances viral clearance during influenza infection.PLoS pathogens · 2014Article
- Article
- The Justy mutant mouse strain produces a spontaneous murine model of salivary gland cancer with myoepithelial and basal cell differentiation.Laboratory investigation; a journal of technical methods and pathology · 2013Article
- SPLUNC1/BPIFA1 contributes to pulmonary host defense against Klebsiella pneumoniae respiratory infection.The American journal of pathology · 2013Article
- T-type amino acid transporter TAT1 (Slc16a10) is essential for extracellular aromatic amino acid homeostasis control.The Journal of physiology · 2012Article
- Dual function of the UNC-45b chaperone with myosin and GATA4 in cardiac development.Journal of cell science · 2012Article
- Generation of N-ethyl-N-nitrosourea (ENU) diabetes models in mice demonstrates genotype-specific action of glucokinase activators.The Journal of biological chemistry · 2011Article
- Selective hippocampal neurodegeneration in transgenic mice expressing small amounts of truncated Aβ is induced by pyroglutamate-Aβ formation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- The isoenzyme of glutaminyl cyclase is an important regulator of monocyte infiltration under inflammatory conditions.EMBO molecular medicine · 2011Article
- Random mutagenesis of the mouse genome: a strategy for discovering gene function and the molecular basis of disease.American journal of physiology. Gastrointestinal and liver physiology · 2011Review
- [Characterization of ENU-mutant mice. Animal models for human diseases using morphological and molecular methods].Der Pathologe · 2010Article
- The Justy mutation identifies Gon4-like as a gene that is essential for B lymphopoiesis.The Journal of experimental medicine · 2010Article
- Dll1 haploinsufficiency in adult mice leads to a complex phenotype affecting metabolic and immunological processes.PloS one · 2009Article
- Towards better mouse models: enhanced genotypes, systemic phenotyping and envirotype modelling.Nature reviews. Genetics · 2009Review
- Improved generation of rat gene knockouts by target-selected mutagenesis in mismatch repair-deficient animals.BMC genomics · 2008Article
- Positional cloning of "Lisch-Like", a candidate modifier of susceptibility to type 2 diabetes in mice.PLoS genetics · 2008Article
- TRESK two-pore-domain K+ channels constitute a significant component of background potassium currents in murine dorsal root ganglion neurones.The Journal of physiology · 2007Article
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Authors and funding
25 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Mice with targeted genetic alterations are the most effective tools for deciphering organismal gene function. We generated an ENU-based parallel C3HeB/FeJ sperm and DNA archive characterized by a high probability to identify allelic variants of target genes as well as high efficiencies in allele retrieval and model revitalization. Our archive size of over 17,000 samples contains approximately 340,000 independent alleles (20 functional mutations per individual sample). Based on an estimated number of approximately 30,000 mouse genes, the parallel sperm/DNA archive should permit the identification and recovery of ten or more alleles per average target gene which translates to a calculated 99% success rate in the discovery of five allelic variants for any given average gene. The low rate of unrelated ENU-induced passenger mutations has no practical impact on the analysis of the allele-specific phenotype at the G3 generation because of dilution and free segregation of such unrelated passenger mutations. To date 39 mouse models representing 33 different genes have been recovered from our archive using in vitro fertilization techniques. The generation time for a murine model heterozygous for a mutation in a gene of interest is less than 2 months, i.e., three to four times faster compared with current embryonic stem-cell-based technologies. We conclude that ENU-based targeted mutagenesis is a powerful tool for the fast and high-throughput production of murine gene-specific models for biomedical research.
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