ArticleProceedings of the National Academy of Sciences of the United States of America2005
Directed evolution of protein switches and their application to the creation of ligand-binding proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
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Who cites it
94 citing papers in PubMed, 218 citations in OpenAlex.
- Artificial allosteric protein switches with machine-learning-designed receptors.Nature biotechnology · 2026Article
- Leveraging Autonomous Biological Oscillators for Directed Evolution of Switchable Proteins.Advances in experimental medicine and biology · 2026Review
- Nickase fidelity drives EvolvR-mediated diversification in mammalian cells.Nature communications · 2025Article
- Lanthanide-Controlled Protein Switches: Development and In Vitro and In Vivo Applications.Angewandte Chemie (International ed. in English) · 2025Article
- ProteinReDiff: Complex-based ligand-binding proteins redesign by equivariant diffusion-based generative models.Structural dynamics (Melville, N.Y.) · 2024Article
- Current status and emerging frontiers in enzyme engineering: An industrial perspective.Heliyon · 2024Review
- Computational design of Periplasmic binding protein biosensors guided by molecular dynamics.PLoS computational biology · 2024Article
- Chimeric Protein Switch Biosensors.Advances in biochemical engineering/biotechnology · 2024Review
- Dissecting the Determinants of Domain Insertion Tolerance and Allostery in Proteins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Protein sensors combining both on-and-off model for antibody homogeneous assay.Biosensors & bioelectronics · 2022Article
- Catalyst-based biomolecular logic gates.Catalysts (Basel, Switzerland) · 2022Article
- Advances in the Computational Design of Small-Molecule-Controlled Protein-Based Circuits for Synthetic Biology.Proceedings of the IEEE. Institute of Electrical and Electronics Engineers · 2022Article
- A central role for amyloid fibrin microclots in long COVID/PASC: origins and therapeutic implications.The Biochemical journal · 2022Review
- Engineering and exploiting synthetic allostery of NanoLuc luciferase.Nature communications · 2022Article
- Article
- Towards an engineering theory of evolution.Nature communications · 2021Review
- FimH as a scaffold for regulated molecular recognition.Journal of biological engineering · 2021Article
- Protease circuits for processing biological information.Nature communications · 2020Article
- Article
- Engineering the hCRBPII Domain-Swapped Dimer into a New Class of Protein Switches.Journal of the American Chemical Society · 2019Article
34 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe an iterative approach for creating protein switches involving the in vitro recombination of two nonhomologous genes. We demonstrate this approach by recombining the genes coding for TEM1 beta-lactamase (BLA) and the Escherichia coli maltose binding protein (MBP) to create a family of MBP-BLA hybrids in which maltose is a positive or negative effector of beta-lactam hydrolysis. Some of these MBP-BLA switches were effectively "on-off" in nature, with maltose altering catalytic activity by as much as 600-fold. The ability of these switches to confer an effector-dependent growth/no growth phenotype to E. coli cells was exploited to rapidly identify, from a library of 4 x 10(6) variants, MBP-BLA switch variants that respond to sucrose as the effector. The transplantation of these mutations into wild-type MBP converted MBP into a "sucrose-binding protein," illustrating the switches potential as a tool to rapidly identify ligand-binding proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.