ArticleThe Journal of cell biology2005
Dynamic formation of RecA filaments at DNA double strand break repair centers in live cells.
Article in The Journal of cell biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 91 citations in OpenAlex.
- How Bioactive Glass S53P4 Kills Bacteria.Journal of functional biomaterials · 2026Article
- Rescuing the bacterial replisome at a nick requires recombinational repair and helicase reloading.Nature communications · 2025Article
- Processing of stalled replication forks in Bacillus subtilis.FEMS microbiology reviews · 2024Review
- ATPase Activity of Bacillus subtilis RecA Affects the Dynamic Formation of RecA Filaments at DNA Double Strand Breaks.mSphere · 2022Article
- Beneficial and detrimental genes in the cellular response to replication arrest.PLoS genetics · 2022Article
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
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- Symmetric activity of DNA polymerases at and recruitment of exonuclease ExoR and of PolA to the Bacillus subtilis replication forks.Nucleic acids research · 2019Article
- Spatial and temporal organization of RecA in theeLife · 2019Article
- A bacterial DNA repair pathway specific to a natural antibiotic.Molecular microbiology · 2019Article
- Single molecule tracking reveals spatio-temporal dynamics of bacterial DNA repair centres.Scientific reports · 2018Article
- Single molecule tracking reveals that the bacterial SMC complex moves slowly relative to the diffusion of the chromosome.Nucleic acids research · 2018Article
- Dynamics of RecA-mediated repair of replication-dependent DNA breaks.The Journal of cell biology · 2018Article
- Between sisters: Watching replication-associated recombinational DNA repair.The Journal of cell biology · 2018Article
- Addressing the Requirements of High-Sensitivity Single-Molecule Imaging of Low-Copy-Number Proteins in Bacteria.Chemphyschem : a European journal of chemical physics and physical chemistry · 2016Article
- Interactions and Localization of Escherichia coli Error-Prone DNA Polymerase IV after DNA Damage.Journal of bacteriology · 2015Article
- Rapid pairing and resegregation of distant homologous loci enables double-strand break repair in bacteria.The Journal of cell biology · 2015Article
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Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We show that RecN protein is recruited to a defined DNA double strand break (DSB) in Bacillus subtilis cells at an early time point during repair. Because RecO and RecF are successively recruited to DSBs, it is now clear that dynamic DSB repair centers (RCs) exist in prokaryotes. RecA protein was also recruited to RCs and formed highly dynamic filamentous structures, which we term threads, across the nucleoids. Formation of RecA threads commenced approximately 30 min after the induction of DSBs, after RecN recruitment to RCs, and disassembled after 2 h. Time-lapse microscopy showed that the threads rapidly changed in length, shape, and orientation within minutes and can extend at 1.02 microm/min. The formation of RecA threads was abolished in recJ addAB mutant cells but not in each of the single mutants, suggesting that RecA filaments can be initiated via two pathways. Contrary to proteins forming RCs, DNA polymerase I did not form foci but was present throughout the nucleoids (even after induction of DSBs or after UV irradiation), suggesting that it continuously scans the chromosome for DNA lesions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.