ArticleNaunyn-Schmiedeberg's archives of pharmacology2005
Effects of ageing on muscarinic receptor subtypes and function in rat urinary bladder.
Article in Naunyn-Schmiedeberg's archives of pharmacology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 55 citations in OpenAlex.
- Choice of y-axis can mislead readers.Naunyn-Schmiedeberg's archives of pharmacology · 2020Article
- Normalization of organ bath contraction data for tissue specimen size: does one approach fit all?Naunyn-Schmiedeberg's archives of pharmacology · 2020Article
- Urinary Bladder Weight and Function in a Rat Model of Mild Hyperglycemia and Its Treatment With Dapagliflozin.Frontiers in pharmacology · 2019Article
- The aging bladder insights from animal models.Asian journal of urology · 2018Review
- Translational Research and Functional Changes in Voiding Function in Older Adults.Clinics in geriatric medicine · 2015Review
- Refractory overactive bladder: a common problem?International urogynecology journal · 2015Review
- Aging changes agonist induced contractile responses in permeabilized rat bladder.Age (Dordrecht, Netherlands) · 2015Article
- Research Findings on Overactive Bladder.Current urology · 2015Review
- Neural control of the lower urinary tract.Comprehensive Physiology · 2015Review
- Voiding dysfunction due to detrusor underactivity: an overview.Nature reviews. Urology · 2014Review
- Loss of adenosine A2B receptor mediated relaxant responses in the aged female rat bladder; effects of dietary phytoestrogens.Naunyn-Schmiedeberg's archives of pharmacology · 2012Article
- Muscarinic receptor subtypes and signalling involved in the attenuation of isoprenaline-induced rat urinary bladder relaxation.Naunyn-Schmiedeberg's archives of pharmacology · 2011Article
- Muscarinic Acetylcholine Receptor M3 Mutation Causes Urinary Bladder Disease and a Prune-Belly-like Syndrome.American journal of human genetics · 2011Article
- Aging differentially modifies agonist-evoked mouse detrusor contraction and calcium signals.Age (Dordrecht, Netherlands) · 2011Article
- Human Urinary Bladder Strip Relaxation by the β-Adrenoceptor Agonist Isoprenaline: Methodological Considerations and Effects of Gender and Age.Frontiers in pharmacology · 2011Article
- Impaired bladder function in aging male rats.The Journal of urology · 2010Article
- Attenuation of age- and sucrose-induced insulin resistance and syndrome X by a synergistic antioxidant cocktail: the AMIS syndrome and HISS hypothesis.Canadian journal of physiology and pharmacology · 2010Review
- Perspectives on overactive bladder in the elderly population.World journal of urology · 2009Review
- Effects of estrogen on age-related changes in muscarinic responsiveness of the urinary bladder and lumbosacral dorsal root ganglion cells in female rats.Molecular and cellular biochemistry · 2008Article
- HISS-dependent insulin resistance (HDIR) in aged rats is associated with adiposity, progresses to syndrome X, and is attenuated by a unique antioxidant cocktail.Experimental gerontology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We compared the density and function of M2 and M3 muscarinic acetylcholine receptor subtypes in the urinary bladder of young adult (3 months) and old (23 months) male Wistar rats. Old rats had a reduced density of muscarinic receptors (96+/-10 vs. 156+/-21 fmol/mg protein), but competition experiments with the M3-selective darifenacin did not indicate alterations in the relative roles of M2 and M3 receptors, with the former being more abundant. The amount of immunodetectable alpha-subunits of various G-proteins potentially linked to muscarinic receptor function was unchanged. The potency of carbachol to contract bladder strips was also unaltered; its maximum effects as well as those of a single KCl concentration were unchanged if raw data or those corrected for strip length were analysed, but somewhat reduced when those corrected for strip weight were analysed. Antagonistic effects of atropine, the M2-selective Ro 320-6206 and the M3-selective darifenacin were unchanged. Agonistic effects of the M3-sparing agonist 4-(2-oxo-2,3-dihydro-benzoimidazol-1-yl)-[1,4']bipiperidinyl-1'-carboxylic acid ethyl ester were similarly poor in young and old rats. Additional experiments were concomitantly performed in submandibular glands from the same animals. While total muscarinic receptor density in submandibular glands was not significantly affected by age (56+/-5 vs. 61+/-4 fmol/mg protein), the relative contribution of M3 receptors significantly declined from 68+/-3% to 57+/-2% based upon darifenacin competition curves. We conclude that aged Wistar rats express fewer muscarinic receptors in their urinary bladder, but there is no change in the relative abundance of M2 and M3 receptors; this is accompanied by only minor if any alterations in receptor responsiveness. In contrast, submandibular gland expresses similar receptor numbers in young and old rats, but slightly fewer M3 receptors in old animals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.