ArticleMolecular and cellular biology2005
Exploiting the keratin 17 gene promoter to visualize live cells in epithelial appendages of mice.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 58 citations in OpenAlex.
- Overactive Wnt5a signaling disrupts hair follicle polarity during mouse skin development.Development (Cambridge, England) · 2022Article
- Transcriptional Differences in Lipid-Metabolizing Enzymes in Murine Sebocytes Derived from Sebaceous Glands of the Skin and Preputial Glands.International journal of molecular sciences · 2021Article
- Cell influx and contractile actomyosin force drive mammary bud growth and invagination.The Journal of cell biology · 2021Article
- Wound healing with topical BRAF inhibitor therapy in a diabetic model suggests tissue regenerative effects.PloS one · 2021Article
- Keratin 17 regulates nuclear morphology and chromatin organization.Journal of cell science · 2020Article
- Skin remodeling and wound healing in the Gottingen minipig following exposure to sulfur mustard.Experimental and molecular pathology · 2020Article
- Live Tissue Imaging Sheds Light on Cell Level Events During Ectodermal Organ Development.Frontiers in physiology · 2020Review
- Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense.Cell stem cell · 2019Article
- Article
- Functional redundancy of frizzled 3 and frizzled 6 in planar cell polarity control of mouse hair follicles.Development (Cambridge, England) · 2018Article
- Developing a sense of touch.Development (Cambridge, England) · 2017Review
- GLI-mediated Keratin 17 expression promotes tumor cell growth through the anti-apoptotic function in oral squamous cell carcinomas.Journal of cancer research and clinical oncology · 2017Article
- p63 exerts spatio-temporal control of palatal epithelial cell fate to prevent cleft palate.PLoS genetics · 2017Article
- Early epithelial signaling center governs tooth budding morphogenesis.The Journal of cell biology · 2016Article
- Foxi3 Deficiency Compromises Hair Follicle Stem Cell Specification and Activation.Stem cells (Dayton, Ohio) · 2016Article
- The spatio-temporal domains of Frizzled6 action in planar polarity control of hair follicle orientation.Developmental biology · 2016Article
- Article
- Ectodysplasin/NF-κB Promotes Mammary Cell Fate via Wnt/β-catenin Pathway.PLoS genetics · 2015Article
- In vivo gene silencing following non-invasive siRNA delivery into the skin using a novel topical formulation.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Piwil2 inhibits keratin 8 degradation through promoting p38-induced phosphorylation to resist Fas-mediated apoptosis.Molecular and cellular biology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Keratin genes afford, given their large number (>50) and differential regulation, a unique opportunity to study the mechanisms underlying specification and differentiation in epithelia of higher metazoans. Moreover, the small size and regulation in cis of many keratin genes enable the use of their regulatory sequence to achieve targeted gene expression in mice. Here we show that 2 kilobases of 5' upstream region from the mouse keratin 17 gene (mK17) confers expression of green fluorescent protein (GFP) in major epithelial appendages of transgenic mice. Like that of mK17, onset of [mK17 5']-GFP reporter expression coincides with the appearance of ectoderm-derived epithelial appendages during embryonic development. In adult mice, [mK17 5']-GFP is appropriately regulated within hair, nail, glands, and oral papilla. Tracking of GFP fluorescence allows for the visualization of growth cycle-related changes in hair follicles, and the defects engendered by the hairless mutation, in live skin tissue. Deletion of an internal 48-bp interval, which encompasses a Gli-responsive element, from this promoter results in loss of GFP fluorescence in most appendages in vivo, suggesting that sonic hedgehog participates in K17 regulation. The compact mK17 gene promoter provides a novel tool for appendage-preferred gene expression and manipulation in transgenic mice.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.