ArticleMolecular and cellular biology2005
HANP1/H1T2, a novel histone H1-like protein involved in nuclear formation and sperm fertility.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 125 citations in OpenAlex.
- The eutherian-specific histone H3.4 promotes germ cell development and reproductive fitness.Nature communications · 2026Article
- SPACA4 regulates the structure and molecular basis of spermatid maturation and ultimately affects sperm quality in mice.Asian journal of andrology · 2026Article
- Dynamic architecture of mammalian paternal chromatin: histone-to-protamine exchange and post-fertilization reprogramming.Epigenetics & chromatin · 2025Review
- Epigenetic regulation by DNA methylation, histone modifications and chromatin remodeling complexes in controlling spermatogenesis and their dysfunction with male infertility.Cellular and molecular life sciences : CMLS · 2025Review
- Article
- Casting histone variants during mammalian reproduction.Chromosoma · 2023Review
- Emerging evidence that the mammalian sperm epigenome serves as a template for embryo development.Nature communications · 2023Review
- A rapidly evolving single copy histone H1 variant is associated with male fertility in a parasitoid wasp.Frontiers in cell and developmental biology · 2023Article
- Loss-of-function mutations inScience advances · 2022Article
- Mouse EWSR1 is crucial for spermatid post-meiotic transcription and spermiogenesis.Development (Cambridge, England) · 2021Article
- The tumour suppressor brain tumour (Brat) regulates linker histone dBigH1 expression in theOpen biology · 2021Article
- Histone variants at a glance.Journal of cell science · 2021Review
- Histone variants in skeletal myogenesis.Epigenetics · 2021Review
- Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids.Epigenetics & chromatin · 2021Article
- Towards Post-Meiotic Sperm Production: Genetic Insight into Human Infertility from Mouse Models.International journal of biological sciences · 2021Review
- Knockout of family with sequence similarity 170 member A (Fam170a) causes male subfertility, while Fam170b is dispensable in mice†.Biology of reproduction · 2020Article
- Essential Role of Histone Replacement and Modifications in Male Fertility.Frontiers in genetics · 2019Review
- Spermatid-specific linker histone HILS1 is a poor condenser of DNA and chromatin and preferentially associates with LINE-1 elements.Epigenetics & chromatin · 2018Article
- Cstf2t Regulates expression of histones and histone-like proteins in male germ cells.Andrology · 2018Article
- The arginine methyltransferase CARM1 represses p300•ACT•CREMτ activity and is required for spermiogenesis.Nucleic acids research · 2018Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We cloned a testis-specific cDNA from mice that encodes a histone H1-like, haploid germ cell-specific nuclear protein designated HANP1/H1T2. The HANP1/H1T2 protein was specifically localized to the nuclei of murine spermatids during differentiation steps 5 to 13 but not to the nuclei of mature sperm. HANP1/H1T2 contains an arginine-serine-rich domain and an ATP/GTP binding site, and it binds to DNA, ATP, and protamine. To investigate the physiological role of HANP1/H1T2, we generated Hanp1/H1T2-disrupted mutant mice. Homozygous Hanp1/H1T2 mutant males were infertile, but females were fertile. Although a substantial number of sperm were recovered from the epididymides, their shape and function were abnormal. During sperm morphogenesis, the formation of nuclei was disturbed and protamine-1 and -2 were only weakly detectable in the nuclei. The chromatin packaging was aberrant, as demonstrated by electron microscopy and biochemical analysis. The mutant sperm exhibited deficient motility and were not competent to fertilize eggs under in vitro fertilization conditions; however, they were capable of fertilizing eggs via intracytoplasmic sperm injection that resulted in the birth of healthy progeny. Thus, we found that HANP1/H1T2 is essential for nuclear formation in functional spermatozoa and is specifically involved in the replacement of histones with protamines during spermiogenesis. At the time of submission of the manuscript, we found an independent publication by Martianov et al. (I. Martianov, S. Brancorsini, R. Catena, A. Gansmuller, N. Kotaja, M. Parvinen, P. Sassone-Corsi, and I. Davidson, Proc. Natl. Acad. Sci. USA 102:2808-2813, 2005) that reported similar results.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.