ArticleMolecular and cellular biology2005
Subunit contributions to histone methyltransferase activities of fly and worm polycomb group complexes.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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Who cites it
99 citing papers in PubMed, 214 citations in OpenAlex.
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- Analysis of polycomb repressive complex 2 (PRC2) subunits in Picea abies with a focus on embryo development.BMC plant biology · 2023Article
- CK2-mediated phosphorylation of SUZ12 promotes PRC2 function by stabilizing enzyme active site.Nature communications · 2022Article
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- Phylogenetic profiling resolves early emergence of PRC2 and illuminates its functional core.Life science alliance · 2022Article
- Evolutionary adaptation of the Polycomb repressive complex 2.Epigenetics & chromatin · 2022Review
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- Epigenetic regulation of drosophila germline stem cell maintenance and differentiation.Developmental biology · 2021Review
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- The Role of Polycomb Group Protein BMI1 in DNA Repair and Genomic Stability.International journal of molecular sciences · 2021Review
- The PRT6 N-degron pathway restricts VERNALIZATION 2 to endogenous hypoxic niches to modulate plant development.The New phytologist · 2021Article
- The Role of the EZH2 and H3K27me3 Expression as a Predictor of Clinical Outcomes in Salivary Duct Carcinoma Patients: A Large-Series Study With Emphasis on the Relevance to the Combined Androgen Blockade and HER2-Targeted Therapy.Frontiers in oncology · 2021Article
- A Structural Perspective on Gene Repression by Polycomb Repressive Complex 2.Sub-cellular biochemistry · 2021Review
- Post-translational modifications of PRC2: signals directing its activity.Epigenetics & chromatin · 2020Review
- Polycomb Repressive Complex 2: a Dimmer Switch of Gene Regulation in Calvarial Bone Development.Current osteoporosis reports · 2020Review
- LSM2-8 and XRN-2 contribute to the silencing of H3K27me3-marked genes through targeted RNA decay.Nature cell biology · 2020Article
- Engaging chromatin: PRC2 structure meets function.British journal of cancer · 2020Review
39 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The ESC-E(Z) complex of Drosophila melanogaster Polycomb group (PcG) repressors is a histone H3 methyltransferase (HMTase). This complex silences fly Hox genes, and related HMTases control germ line development in worms, flowering in plants, and X inactivation in mammals. The fly complex contains a catalytic SET domain subunit, E(Z), plus three noncatalytic subunits, SU(Z)12, ESC, and NURF-55. The four-subunit complex is >1,000-fold more active than E(Z) alone. Here we show that ESC and SU(Z)12 play key roles in potentiating E(Z) HMTase activity. We also show that loss of ESC disrupts global methylation of histone H3-lysine 27 in fly embryos. Subunit mutations identify domains required for catalytic activity and/or binding to specific partners. We describe missense mutations in surface loops of ESC, in the CXC domain of E(Z), and in the conserved VEFS domain of SU(Z)12, which each disrupt HMTase activity but preserve complex assembly. Thus, the E(Z) SET domain requires multiple partner inputs to produce active HMTase. We also find that a recombinant worm complex containing the E(Z) homolog, MES-2, has robust HMTase activity, which depends upon both MES-6, an ESC homolog, and MES-3, a pioneer protein. Thus, although the fly and mammalian PcG complexes absolutely require SU(Z)12, the worm complex generates HMTase activity from a distinct partner set.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.