ArticleProceedings of the National Academy of Sciences of the United States of America2005
Adult mice cloned from migrating primordial germ cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Mouse somatic cell nuclear transfer: What has changed and remained unchanged in 25 years.The Journal of reproduction and development · 2023Article
- The Evolutionary Advantage in Mammals of the Complementary Monoallelic Expression Mechanism of Genomic Imprinting and Its Emergence From a Defense Against the Insertion Into the Host Genome.Frontiers in genetics · 2022Review
- Technical advances contribute to the study of genomic imprinting.PLoS genetics · 2019Review
- Retinoic acid-stimulated ERK1/2 pathway regulates meiotic initiation in cultured fetal germ cells.PloS one · 2019Article
- Contributions of Ryuzo Yanagimachi to the field of reproductive biology.Biology of reproduction · 2019Article
- Deleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming.Genome biology · 2015Article
- Gene expression and DNA methylation status of chicken primordial germ cells.Molecular biotechnology · 2013Article
- High-resolution DNA methylome analysis of primordial germ cells identifies gender-specific reprogramming in mice.Genome research · 2013Article
- Epigenetic programming and reprogramming during development.Nature structural & molecular biology · 2013Review
- Environmentally induced transgenerational epigenetic reprogramming of primordial germ cells and the subsequent germ line.PloS one · 2013Article
- Bisphenol A exposure modifies DNA methylation of imprint genes in mouse fetal germ cells.Molecular biology reports · 2012Article
- Global profiling of DNA methylation erasure in mouse primordial germ cells.Genome research · 2012Article
- Male differentiation of germ cells induced by embryonic age-specific Sertoli cells in mice.Biology of reproduction · 2012Article
- Non-genetic contributions of the sperm nucleus to embryonic development.Asian journal of andrology · 2011Review
- Unfaithful maintenance of methylation imprints due to loss of maternal nuclear Dnmt1 during somatic cell nuclear transfer.PloS one · 2011Article
- Direct effects of retinoic acid on entry of fetal male germ cells into meiosis in mice.Biology of reproduction · 2010Article
- Autonomous silencing of the imprinted Cdkn1c gene in stem cells.Epigenetics · 2010Article
- Derivation of primordial germ cells from human embryonic and induced pluripotent stem cells is significantly improved by coculture with human fetal gonadal cells.Stem cells (Dayton, Ohio) · 2009Article
- Dynamic nuclear organization of constitutive heterochromatin during fetal male germ cell development in mice.Biology of reproduction · 2009Article
- Small RNA guides for de novo DNA methylation in mammalian germ cells.Genes & development · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
We previously reported that the genomes of gonadal germ cells at 11.5-19.5 days postcoitum (dpc) are incompetent to support full-term development of cloned mouse embryos. In this study, we performed nuclear transfer using primordial germ cells (PGCs) from earlier stages at 8.5-10.5 dpc. When PGC nuclei at 8.5, 9.5, and 10.5 dpc were transferred into enucleated oocytes, seven cloned embryos developed into full-term offspring. Of these, five, all derived from 8.5- or 9.5-dpc PGCs, developed into healthy adults with normal fertility. Of the remaining two offspring derived from 10.5-dpc PGCs, one died shortly after birth, and the other showed slight growth retardation but subsequently developed into a fertile adult. We examined allele-specific methylation at the imprinted H19 and Snrpn loci in 9.5- to 11.5-dpc PGCs. Although the beginning of methylation erasure was evident on the H19 paternal allele at 9.5 dpc, most PGCs did not demonstrate significant erasure of paternal allele-specific methylation until 10.5 dpc. Maternal allele-specific methylation was largely erased from Snrpn by 10.5 dpc. By 11.5 dpc, the majority of PGCs showed nearly complete or complete erasure of allele-specific methylation in both H19 and Snrpn. These results demonstrate that at least some genomic imprints remain largely intact in 8.5- to 9.5-dpc PGCs and then undergo erasure at approximately 10.5 dpc as the PGCs enter the genital ridges. Thus, migrating PGCs at 8.5-9.5 dpc can be successfully used as donors for nuclear transfer, whereas gonadal PGCs at 11.5 dpc and later are incompetent to support full-term development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.