ArticleBiophysical journal2005
Determining protease activity in vivo by fluorescence cross-correlation analysis.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 72 citations in OpenAlex.
- Programmed cell death detection methods: a systematic review and a categorical comparison.Apoptosis : an international journal on programmed cell death · 2022Pooled it
- The ganglioside GM1a functions as a coreceptor/attachment factor for dengue virus during infection.The Journal of biological chemistry · 2022Article
- Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo.Histochemistry and cell biology · 2020Review
- Impact of ConcanavalinA affinity in the intracellular fate of Protein Corona on Glucosamine Au nanoparticles.Scientific reports · 2018Article
- There and back again: from the origin of life to single molecules.European biophysics journal : EBJ · 2018Article
- Proteolytic activity assayed by subcellular localization switching of a substrate.Biochemistry and biophysics reports · 2016Article
- ARHGEF17 is an essential spindle assembly checkpoint factor that targets Mps1 to kinetochores.The Journal of cell biology · 2016Article
- Live-cell observation of cytosolic HIV-1 assembly onset reveals RNA-interacting Gag oligomers.The Journal of cell biology · 2015Article
- Dynamics of the ternary complex formed by c-Myc interactor JPO2, transcriptional co-activator LEDGF/p75, and chromatin.The Journal of biological chemistry · 2014Article
- A CENP-S/X complex assembles at the centromere in S and G2 phases of the human cell cycle.Open biology · 2014Article
- Dynamics of caspase-3 activation and inhibition in embryonic micromasses evaluated by a photon-counting chemiluminescence approach.In vitro cellular & developmental biology. Animal · 2012Article
- Factors affecting the quantification of biomolecular interactions by fluorescence cross-correlation spectroscopy.Biophysical journal · 2012Article
- Step-wise assembly, maturation and dynamic behavior of the human CENP-P/O/R/Q/U kinetochore sub-complex.PloS one · 2012Article
- Proteins on the move: insights gained from fluorescent protein technologies.Nature reviews. Molecular cell biology · 2011Review
- Comparison of protein behavior between wild-type and G601S hERG in living cells by fluorescence correlation spectroscopy.The journal of physiological sciences : JPS · 2011Article
- Fluorescence correlation spectroscopy: a review of biochemical and microfluidic applications.Applied spectroscopy · 2011Review
- Multiple Escherichia coli RecQ helicase monomers cooperate to unwind long DNA substrates: a fluorescence cross-correlation spectroscopy study.The Journal of biological chemistry · 2010Article
- Nuclear import and assembly of influenza A virus RNA polymerase studied in live cells by fluorescence cross-correlation spectroscopy.Journal of virology · 2010Article
- Determination of dissociation constants in living zebrafish embryos with single wavelength fluorescence cross-correlation spectroscopy.Biophysical journal · 2009Article
- Analysis of membrane-localized binding kinetics with FRAP.European biophysics journal : EBJ · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To date, most biochemical approaches to unravel protein function have focused on purified proteins in vitro. Whereas they analyze enzyme performance under assay conditions, they do not necessarily tell us what is relevant within a living cell. Ideally, cellular functions should be examined in situ. In particular, association/dissociation reactions are ubiquitous, but so far there is no standard technique permitting online analysis of these processes in vivo. Featuring single-molecule sensitivity combined with intrinsic averaging, fluorescence correlation spectroscopy is a minimally invasive technique ideally suited to monitor proteins. Moreover, endogenous fluorescence-based assays can be established by genetically encoding fusions of autofluorescent proteins and cellular proteins, thus avoiding the disadvantages of in vitro protein labeling and subsequent delivery to cells. Here, we present an in vivo protease assay as a model system: Green and red autofluorescent proteins were connected by Caspase-3- sensitive and insensitive protein linkers to create double-labeled protease substrates. Then, dual-color fluorescence cross-correlation spectroscopy was employed to study the protease reaction in situ. Allowing assessment of multiple dynamic parameters simultaneously, this method provided internal calibration and improved experimental resolution for quantifying protein stability. This approach, which is easily extended to reversible protein-protein interactions, seems very promising for elucidating intracellular protein functions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.