ArticleBiophysical journal2005
Histamine effects on endothelial cell fibronectin interaction studied by atomic force microscopy.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 76 citations in OpenAlex.
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- Modulation of arterial intima stiffness by disturbed blood flow.Experimental biology and medicine (Maywood, N.J.) · 2024Article
- Smooth Muscle-Alpha Actin R149C Pathogenic Variant Downregulates Integrin Recruitment at Cell-Matrix Adhesions and Decreases Cellular Contractility.International journal of molecular sciences · 2023Article
- Age-Associated Dysregulation of Integrin Function in Vascular Smooth Muscle.Frontiers in physiology · 2022Article
- A Calcium Mediated Mechanism Coordinating Vascular Smooth Muscle Cell Adhesion During KCl Activation.Frontiers in physiology · 2018Article
- Vascular Smooth Muscle Contractile Function Declines With Age in Skeletal Muscle Feed Arteries.Frontiers in physiology · 2018Article
- Nonenzymatic glycation interferes with fibronectin-integrin interactions in vascular smooth muscle cells.Microcirculation (New York, N.Y. : 1994) · 2017Article
- Endothelial Mineralocorticoid Receptor Mediates Diet-Induced Aortic Stiffness in Females.Circulation research · 2016Article
- Atomic Force Microscopy Protocol for Measurement of Membrane Plasticity and Extracellular Interactions in Single Neurons in Epilepsy.Frontiers in aging neuroscience · 2016Article
- "Smooth Muscle Cell Stiffness Syndrome"-Revisiting the Structural Basis of Arterial Stiffness.Frontiers in physiology · 2015Review
- Smooth muscle hyperplasia due to loss of smooth muscle α-actin is driven by activation of focal adhesion kinase, altered p53 localization and increased levels of platelet-derived growth factor receptor-β.Human molecular genetics · 2013Article
- Measurement of the Interaction Between Recombinant I-domain from Integrin alpha 2 beta 1 and a Triple Helical Collagen Peptide with the GFOGER Binding Motif Using Molecular Force Spectroscopy.International journal of molecular sciences · 2013Article
- Amyloid-β peptide on sialyl-Lewis(X)-selectin-mediated membrane tether mechanics at the cerebral endothelial cell surface.PloS one · 2013Article
- Coordination of fibronectin adhesion with contraction and relaxation in microvascular smooth muscle.Cardiovascular research · 2012Article
- Integrin clustering in two and three dimensions.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Effects of Amyloid Beta Peptide on Neurovascular Cells.Central Asian journal of global health · 2012Review
- Cardiomyocyte contractile status is associated with differences in fibronectin and integrin interactions.American journal of physiology. Heart and circulatory physiology · 2010Article
- Membrane biophysics and mechanics in Alzheimer's disease.Molecular neurobiology · 2010Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Atomic force microscopy was used to investigate the cellular response to histamine, one of the major inflammatory mediators that cause endothelial hyperpermeability and vascular leakage. AFM probes were labeled with fibronectin and used to measure binding strength between alpha5beta1 integrin and fibronectin by quantifying the force required to break single fibronectin-integrin bonds. The cytoskeletal changes, binding probability, and adhesion force before and after histamine treatment on endothelial cells were monitored. Cell topography measurements indicated that histamine induces cell shrinkage. Local cell stiffness and binding probability increased twofold after histamine treatment. The force necessary to rupture single alpha5beta1-fibronectin bond increased from 34.0 +/- 0.5 pN in control cells to 39 +/- 1 pN after histamine treatment. Experiments were also conducted to confirm the specificity of the alpha5beta1-fibronectin interaction. In the presence of soluble GRGDdSP the probability of adhesion events decreased >50% whereas the adhesion force between alpha5beta1 and fibronectin remained unchanged. These data indicate that extracellular matrix-integrin interactions play an important role in the endothelial cell response to changes of external chemical mediators. These changes can be recorded as direct measurements on live endothelial cells by using atomic force microscopy.
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