ArticleBiophysical journal2005
Liquid-like water confined in stacks of biological membranes at 200 k and its relation to protein dynamics.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Structure and dynamics of supercooled water in the hydration layer of poly(ethylene glycol).Structural dynamics (Melville, N.Y.) · 2022Article
- Behavior of Hydrated Lipid Bilayers at Cryogenic Temperatures.Frontiers in chemistry · 2020Article
- High protein flexibility and reduced hydration water dynamics are key pressure adaptive strategies in prokaryotes.Scientific reports · 2016Article
- Strong Static Magnetic Fields Increase the Gel Signal in Partially Hydrated DPPC/DMPC Membranes.Membranes · 2015Article
- Derivation and assessment of phase-shifted, disordered vector field models for frustrated solvent interactions.The Journal of chemical physics · 2013Article
- Temperature-dependent macromolecular X-ray crystallography.Acta crystallographica. Section D, Biological crystallography · 2010Article
- Coupled relaxations at the protein-water interface in the picosecond time scale.Journal of the Royal Society, Interface · 2009Article
- Evidence for liquid water during the high-density to low-density amorphous ice transition.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Switch from conventional to distributed kinetics in the bacteriorhodopsin photocycle.Biochemistry · 2008Article
- Dynamics of hydration water in deuterated purple membranes explored by neutron scattering.European biophysics journal : EBJ · 2008Article
- Coupling of protein and hydration-water dynamics in biological membranes.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Solvation effect of bacteriochlorophyll excitons in light-harvesting complex LH2.Biophysical journal · 2007Article
- Neutron scattering reveals extremely slow cell water in a Dead Sea organism.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Conditioning action of the environment on the protein dynamics studied through elastic neutron scattering.European biophysics journal : EBJ · 2006Article
- Controlling the protein dynamical transition with sugar-based bioprotectant matrices: a neutron scattering study.Biophysical journal · 2006Article
- Dielectric and calorimetric studies of hydrated purple membrane.Biophysical journal · 2005Article
- Infrared monitoring of interlayer water in stacks of purple membranes.Photochemistry and photobiologyArticle
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Confined water is of considerable current interest owing to its biophysical importance and relevance to cryopreservation. It can be studied in its amorphous or supercooled state in the "no-man's land", i.e., in the temperature range between 150 and 235 K, in which bulk water is always crystalline. Amorphous deuterium oxide (D(2)O) was obtained in the intermembrane spaces of a stack of purple membranes from Halobacterium salinarum by flash cooling to 77 K. Neutron diffraction showed that upon heating to 200 K the intermembrane water space decreased sharply with an associated strengthening of ice diffraction, indicating that water beyond the first membrane hydration layer flowed out of the intermembrane space to form crystalline ice. It was concluded that the confined water undergoes a glass transition at or below 200 K to adopt an ultraviscous liquid state from which it crystallizes to form ice as soon as it finds itself in an unconfined, bulk-water environment. Our results provide model-free evidence for translational diffusion of confined water in the no-man's land. Potential effects of the confined-water glass transition on nanosecond membrane dynamics were investigated by incoherent elastic neutron scattering experiments. These revealed no differences between flash-cooled and slow-cooled samples (in the latter, the intermembrane space at temperatures <250 K is occupied only by the first membrane hydration layers), with dynamical transitions at 150 and 260 K, but not at 200 K, suggesting that nanosecond membrane dynamics are not sensitive to the state of the water beyond the first hydration shell at cryotemperatures.
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