ArticleMolecular biology of the cell2005
Phosphoproteome profiling of transforming growth factor (TGF)-beta signaling: abrogation of TGFbeta1-dependent phosphorylation of transcription factor-II-I (TFII-I) enhances cooperation of TFII-I and Smad3 in transcription.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- Integrated Phosphoproteomics Identifies TGFβ-Dependent Phosphorylation Events Linking Kinase Signaling to Autophagy in Palatogenesis.Proteomes · 2026Article
- TFII-I-mediated polymerase pausing antagonizes GLI2 induction by TGFβ.Nucleic acids research · 2020Article
- Identification of additional loci associated with antibody response to Mycobacterium avium ssp. Paratuberculosis in cattle by GSEA-SNP analysis.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Article
- Article
- Review
- A phosphoproteomic approach towards the understanding of the role of TGF-β in Trypanosoma cruzi biology.PloS one · 2012Article
- Proteome profiling of heat shock of human primary breast epithelial cells, a dataset report.Cell stress & chaperones · 2011Article
- 2D difference gel electrophoresis analysis of different time points during the course of neoplastic transformation of human mammary epithelial cells.Journal of proteome research · 2011Article
- A crystal structure of the cyclic GMP-dependent protein kinase I{beta} dimerization/docking domain reveals molecular details of isoform-specific anchoring.The Journal of biological chemistry · 2010Article
- Molecular signatures of the primitive prostate stem cell niche reveal novel mesenchymal-epithelial signaling pathways.PloS one · 2010Article
- Hypoxia-activated Smad3-specific dephosphorylation by PP2A.The Journal of biological chemistry · 2010Article
- Understanding protein phosphorylation on a systems level.Briefings in functional genomics · 2010Review
- Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport.Nature structural & molecular biology · 2009Article
- Prediction of acute multiple sclerosis relapses by transcription levels of peripheral blood cells.BMC medical genomics · 2009Article
- Novel crosstalk to BMP signalling: cGMP-dependent kinase I modulates BMP receptor and Smad activity.The EMBO journal · 2009Article
- Identification of the TFII-I family target genes in the vertebrate genome.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transforming growth factor-beta (TGFbeta) signaling involves activation of a number of signaling pathways, several of which are controlled by phosphorylation events. Here, we describe a phosphoproteome profiling of MCF-7 human breast epithelial cells treated with TGFbeta1. We identified 32 proteins that change their phosphorylation upon treatment with TGFbeta1; 26 of these proteins are novel targets of TGFbeta1. We show that Smad2 and Smad3 have different effects on the dynamics of TGFbeta1-induced protein phosphorylation. The identified proteins belong to nine functional groups, e.g., proteins regulating RNA processing, cytoskeletal rearrangements, and proteasomal degradation. To evaluate the proteomics findings, we explored the functional importance of TGFbeta1-dependent phosphorylation of one of the targets, i.e., transcription factor-II-I (TFII-I). We confirmed that TGFbeta1 stimulated TFII-I phosphorylation at serine residues 371 and 743. Abrogation of the phosphorylation by replacement of Ser371 and Ser743 with alanine residues resulted in enhanced complex formation between TFII-I and Smad3, and enhanced cooperation between TFII-I and Smad3 in transcriptional regulation, as evaluated by a microarray-based measurement of expression of endogenous cyclin D2, cyclin D3, and E2F2 genes, and by a luciferase reporter assay. Thus, TGFbeta1-dependent phosphorylation of TFII-I may modulate TGFbeta signaling at the transcriptional level.
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