ArticlePlanta2005
Biochemical properties of isoprene synthase in poplar (Populus x canescens).
Article in Planta, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 91 citations in OpenAlex.
- Isopentenyl diphosphate isomerase exerts limited control over terpenoid biosynthesis in two woody plant species.Plant physiology · 2026Article
- Engineering Cyanobacteria for High-Yield Photosynthetic Isoprene Production With Long-Term Phenotypic Stability.Plant biotechnology journal · 2026Article
- Cryptic isoprene emission of soybeans.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- De Novo Terpenes Emitted from Juvenile Leaves ofMolecules (Basel, Switzerland) · 2025Article
- Deoxyxylulose 5-Phosphate Synthase Does Not Play a Major Role in Regulating the Methylerythritol 4-Phosphate Pathway in Poplar.International journal of molecular sciences · 2024Article
- Optimization of IspSApplied and environmental microbiology · 2023Article
- Emission Trade-Off between Isoprene and Other BVOC Components inInternational journal of molecular sciences · 2023Article
- Microbial Production Potential ofMicroorganisms · 2022Review
- Isoprene enhances leaf cytokinin metabolism and induces early senescence.The New phytologist · 2022Article
- Protein expression plasticity contributes to heat and drought tolerance of date palm.Oecologia · 2021Article
- Validation of an insertion-engineered isoprene synthase as a strategy to functionalize terpene synthases.RSC advances · 2021Article
- Evolution of isoprene emission in Arecaceae (palms).Evolutionary applications · 2021Article
- Anaerobic Production of Isoprene by EngineeredApplied and environmental microbiology · 2021Article
- Isoprene Exposure in the United States Based on Urinary IPM3: NHANES 2015-2016.Environmental science & technology · 2020Article
- Rapid conversion of isoprene photooxidation products in terrestrial plants.Communications earth & environment · 2020Article
- Isoprene Acts as a Signaling Molecule in Gene Networks Important for Stress Responses and Plant Growth.Plant physiology · 2019Article
- Metabolic engineering for the production of isoprene and isopentenol by Escherichia coli.Applied microbiology and biotechnology · 2018Review
- Bio-production of gaseous alkenes: ethylene, isoprene, isobutene.Biotechnology for biofuels · 2018Review
- Structural and Chemical Biology of Terpenoid Cyclases.Chemical reviews · 2017Review
- Controls of the quantum yield and saturation light of isoprene emission in different-aged aspen leaves.Plant, cell & environment · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isoprene synthase (ISPS) catalyzes the elimination of pyrophosphate from dimethylallyl diphosphate (DMADP) forming isoprene, a volatile hydrocarbon emitted from many plant species to the atmosphere. In the present work, immunological techniques were applied to study and localize ISPS in poplar leaves (Populus x canescens). Immunogold labeling using polyclonal antibodies generated against His-tagged recombinant ISPS protein detected ca. 44% of ISPS in the stroma of the chloroplasts and ca. 56% of gold particles attached to the stromal-facing side of the thylakoid membranes. ISPS isolated from leaves exhibited the same biochemical properties as the recombinant ISPS without the plastid-targeting peptide heterologous expressed in E. coli, whereas an additional C- or N-terminal His-tag changed the biochemical features of the recombinant enzyme with regard to temperature, pH, and substrate dependence. In comparison to the closely related class of monoterpene synthases from angiosperms and ISPS of oaks, the most striking feature of the poplar ISPS is a cooperative substrate dependence which is characteristic to enzymes with positive substrate activation. The detection of four immunoreactive bands in poplar leaf extracts with isoelectric points from 5.0 to 5.5 and a native molecular weight of ca. 51 kDa give reason for future studies on post-translational modifications of ISPS.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.