ArticleBritish journal of cancer2005
Comparison of hypoxia transcriptome in vitro with in vivo gene expression in human bladder cancer.
Article in British journal of cancer, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 61 citations in OpenAlex.
- SLC16A3 links tumor metabolism to therapeutic opportunities in hepatocellular carcinoma.Translational cancer research · 2026Review
- Gene Expression in Muscle-Invasive and Non-Muscle-Invasive Bladder Cancer Cells Exposed to Hypoxia.Cancers · 2025Article
- Understanding metabolic alterations and heterogeneity in cancer progression through validated immunodetection of key molecular components: a case of carbonic anhydrase IX.Cancer metastasis reviews · 2021Review
- Level of Murine DDX3 RNA Helicase Determines Phenotype Changes of Hepatocytes In Vitro and In Vivo.International journal of molecular sciences · 2021Article
- Development of NovelJournal of medicinal chemistry · 2021Article
- Impairment of carbonic anhydrase IX ectodomain cleavage reinforces tumorigenic and metastatic phenotype of cancer cells.British journal of cancer · 2020Article
- Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models.Frontiers in endocrinology · 2020Review
- Ultrasound beam steering of oxygen nanobubbles for enhanced bladder cancer therapy.Scientific reports · 2018Article
- Hypoxia-mediated upregulation of MCT1 expression supports the glycolytic phenotype of glioblastomas.Oncotarget · 2016Article
- MCT4 as a potential therapeutic target for metastatic gastric cancer with peritoneal carcinomatosis.Oncotarget · 2016Article
- Increased S100A4 expression in the vasculature of human COPD lungs and murine model of smoke-induced emphysema.Respiratory research · 2015Article
- Prolonged hypoxia induces monocarboxylate transporter-4 expression in mesenchymal stem cells resulting in a secretome that is deleterious to cardiovascular repair.Stem cells (Dayton, Ohio) · 2015Article
- Tumor-stroma metabolic relationship based on lactate shuttle can sustain prostate cancer progression.BMC cancer · 2014Article
- Exploring the miRNA-mRNA regulatory network in clear cell renal cell carcinomas by next-generation sequencing expression profiles.BioMed research international · 2014Article
- HERV-K and LINE-1 DNA Methylation and Reexpression in Urothelial Carcinoma.Frontiers in oncology · 2013Article
- Antenatal maternal long-term hypoxia: acclimatization responses with altered gene expression in ovine fetal carotid arteries.PloS one · 2013Article
- RNA interference targeting adrenomedullin induces apoptosis and reduces the growth of human bladder urothelial cell carcinoma.Medical oncology (Northwood, London, England) · 2013Article
- Hypoxia-induced endothelial CX3CL1 triggers lung smooth muscle cell phenotypic switching and proliferative expansion.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- New perspectives for in vitro risk assessment of multiwalled carbon nanotubes: application of coculture and bioinformatics.Journal of toxicology and environmental health. Part B, Critical reviews · 2012Article
- Prognostic significance of monocarboxylate transporter 4 expression in patients with colorectal cancer.Experimental and therapeutic medicine · 2012Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hypoxia-inducible genes have been linked to the aggressive phenotype of cancer. However, nearly all work on hypoxia-regulated genes has been conducted in vitro on cell lines. We investigated the hypoxia transcriptome in primary human bladder cancer using cDNA microarrays to compare genes induced by hypoxia in vitro in bladder cancer cell line EJ28 with genes upregulated in 39 bladder tumour specimens (27 superficial and 12 invasive). We correlated array mRNA fold changes with carbonic anhydrase 9 (CA IX) staining of tumours as a surrogate marker of hypoxia. Of 6000 genes, 32 were hypoxia inducible in vitro more than two-fold, five of which were novel, including lactate transporter SLC16A3 and RNAse 4. Eight of 32 hypoxia-inducible genes in vitro were also upregulated on the vivo array. Vascular endothelial growth factor mRNA was upregulated two-fold by hypoxia and 2-18-fold in 31 out of 39 tumours. Glucose transporter 1 was also upregulated on both arrays mRNA, and fold changes on the in vivo array significantly correlated with CA IX staining of tumours (P=0.008). However, insulin-like growth factor binding protein 3 mRNA was the most strongly differentially expressed gene in both arrays and this confirmed its upregulation in urine of bladder cancer patients (n=157, P<0.01). This study defines genes suitable for an in vivo hypoxia 'profile', shows the heterogeneity of the hypoxia response and describes new hypoxia-regulated genes.
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