ArticleJournal of biosciences2005
Detergent-resistant membranes in human erythrocytes and their connection to the membrane-skeleton.
Article in Journal of biosciences, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 56 citations in OpenAlex.
- Stomatin and Stomatin-Like Proteins Can Regulate Transporter Proteins Activity and Has a Role in Cancer Metastasis.The Journal of membrane biology · 2025Review
- Role of Plasma Membrane at Dielectric Relaxations and Intermembrane Interaction in Human Erythrocytes.Membranes · 2023Article
- Identification and preliminary characterization of Plasmodium falciparum proteins secreted upon gamete formation.Scientific reports · 2022Article
- Glycosphingolipid GM3 is localized in both exoplasmic and cytoplasmic leaflets of Plasmodium falciparum malaria parasite plasma membrane.Scientific reports · 2021Article
- Membrane Rearrangements in the Maturation of Circulating Human Reticulocytes.Frontiers in physiology · 2020Article
- Continuous Change in Membrane and Membrane-Skeleton Organization During Development From Proerythroblast to Senescent Red Blood Cell.Frontiers in physiology · 2018Review
- Proteomics of Stored Red Blood Cell Membrane and Storage-Induced Microvesicles Reveals the Association of Flotillin-2 With Band 3 Complexes.Frontiers in physiology · 2018Article
- Biophysical approaches in the study of biomembrane solubilization: quantitative assessment and the role of lateral inhomogeneity.Biophysical reviews · 2017Review
- Proteomic analysis of detergent-resistant membrane microdomains in trophozoite blood stage of the human malaria parasite Plasmodium falciparum.Molecular & cellular proteomics : MCP · 2013Article
- Article
- Palmitoylation of MPP1 (membrane-palmitoylated protein 1)/p55 is crucial for lateral membrane organization in erythroid cells.The Journal of biological chemistry · 2012Article
- Effect of cholesterol depletion and temperature on the isolation of detergent-resistant membranes from human erythrocytes.The Journal of membrane biology · 2010Article
- Formation of two different types of ion channels by amphotericin B in human erythrocyte membranes.The Journal of membrane biology · 2009Article
- Altered membrane structure and surface potential in homozygous hemoglobin C erythrocytes.PloS one · 2009Article
- Resistance of human erythrocyte membranes to Triton X-100 and C12E8.The Journal of membrane biology · 2009Article
- Electron Pathways through Erythrocyte Plasma Membrane in Human Physiology and Pathology: Potential Redox Biomarker?Biomarker insights · 2007Article
- Article
- The role of seladin-1/DHCR24 in cholesterol biosynthesis, APP processing and Abeta generation in vivo.The EMBO journal · 2006Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In cell membranes, local inhomogeneity in the lateral distribution of lipids and proteins is thought to exist in vivo in the form of lipid 'rafts', microdomains enriched in cholesterol and sphingolipids, and in specific classes of proteins, that appear to play specialized roles for signal transduction, cell-cell recognition, parasite or virus infection, and vesicular trafficking. These structures are operationally defined as membranes resistant to solubilization by nonionic detergents at 4 degree C (detergent-resistant membranes, DRMs). This definition appears to be necessary and sufficient, although additional manoeuvres, not always described with sufficient detail, may be needed to ensure isolation of DRMs, like mechanical homogenization, and changes in the pH and/or ionic strength of the solubilization medium. We show here for the human erythrocyte that the different conditions adopted may lead to the isolation of qualitatively and quantitatively different DRM fractions, thus contributing to the complexity of the notion itself of lipid raft. A significant portion of erythrocyte DRMs enriched in reported lipid raft markers, such as flotillin-1, flotillin-2 and GM1, is anchored to the spectrin membrane-skeleton via electrostatic interactions that can be disrupted by the simultaneous increase in pH and ionic strength of the solubilization medium.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.