ArticleNucleic acids research2005
Assigning functions to genes: identification of S-phase expressed genes in Leishmania major based on post-transcriptional control elements.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Synchronization of Leishmania amazonensis Cell Cycle Using Hydroxyurea.Methods in molecular biology (Clifton, N.J.) · 2022Article
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- Substrate channeling between the human dihydrofolate reductase and thymidylate synthase.Protein science : a publication of the Protein Society · 2016Article
- eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei.RNA (New York, N.Y.) · 2014Article
- Ubiquitination-mediated interaction among domains is responsible for inhibition of RNA endonuclease activity of mRNA cycling sequence binding protein from L. donovani (LdCSBP).Parasitology research · 2014Article
- Identification of the HSP70-II gene in Leishmania braziliensis HSP70 locus: genomic organization and UTRs characterization.Parasites & vectors · 2011Article
- Article
- A mitochondrial DNA primase is essential for cell growth and kinetoplast DNA replication in Trypanosoma brucei.Molecular and cellular biology · 2010Article
- Trypanosoma brucei PUF9 regulates mRNAs for proteins involved in replicative processes over the cell cycle.PLoS pathogens · 2009Article
- Article
- Key role of the 3' untranslated region in the cell cycle regulated expression of the Leishmania infantum histone H2A genes: minor synergistic effect of the 5' untranslated region.BMC molecular biology · 2009Article
- Activity of hydroxyurea against Leishmania mexicana.Antimicrobial agents and chemotherapy · 2008Article
- Effect of hydroxyurea on procyclic Trypanosoma brucei: an unconventional mechanism for achieving synchronous growth.Eukaryotic cell · 2008Article
- Coordinate regulation of a family of promastigote-enriched mRNAs by the 3'UTR PRE element in Leishmania mexicana.Molecular and biochemical parasitology · 2008Article
- Mitochondrial origin-binding protein UMSBP mediates DNA replication and segregation in trypanosomes.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Identification of new kinetoplast DNA replication proteins in trypanosomatids based on predicted S-phase expression and mitochondrial targeting.Eukaryotic cell · 2007Article
- Cell cycle-dependent localization and properties of a second mitochondrial DNA ligase in Crithidia fasciculata.Eukaryotic cell · 2006Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Assigning functions to genes is one of the major challenges of the post-genomic era. Usually, functions are assigned based on similarity of the coding sequences to sequences of known genes, or by identification of transcriptional cis-regulatory elements that are known to be associated with specific pathways or conditions. In trypanosomatids, where regulation of gene expression takes place mainly at the post-transcriptional level, new approaches for function assignment are needed. Here we demonstrate the identification of novel S-phase expressed genes in Leishmania major, based on a post-transcriptional control element that was recognized in Crithidia fasciculata as involved in the cell cycle-dependent expression of several nuclear and mitochondrial S-phase expressed genes. Hypothesizing that a similar regulatory mechanism is manifested in L.major, we have applied a computational search for similar control elements in the genome of L.major. Our computational scan yielded 132 genes, of which 33% are homologues of known DNA metabolism genes and 63% lack any annotation. Experimental testing of seven of these genes revealed that their mRNAs cycle throughout the cell cycle, reaching a maximum level during S-phase or just prior to it. It is suggested that screening for post-transcriptional control elements associated with a specific function provides an efficient method for assigning functions to trypanosomatid genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.