ArticleJournal of virology2005
Ebola virus VP40 late domains are not essential for viral replication in cell culture.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Exploring the druggability of the UEV domain of human TSG101 in search for broad-spectrum antivirals.Protein science : a publication of the Protein Society · 2025Article
- Molecular insights into the Ebola virus life cycle.Nature microbiology · 2024Review
- The Mucin-Like Domain of the Ebola Glycoprotein Does Not Impact Virulence or Pathogenicity in Ferrets.The Journal of infectious diseases · 2023Article
- Elucidating Residue-Level Determinants Affecting Dimerization of Ebola Virus Matrix Protein Using High-Throughput Site Saturation Mutagenesis and Biophysical Approaches.The journal of physical chemistry. B · 2023Article
- Assessment of Life Cycle Modeling Systems as Prediction Tools for a Possible Attenuation of Recombinant Ebola Viruses.Viruses · 2022Article
- Structural and Functional Aspects of Ebola Virus Proteins.Pathogens (Basel, Switzerland) · 2021Review
- Review
- Budding of Ebola Virus Particles Requires the Rab11-Dependent Endocytic Recycling Pathway.The Journal of infectious diseases · 2018Article
- Chaperone-Mediated Autophagy Protein BAG3 Negatively Regulates Ebola and Marburg VP40-Mediated Egress.PLoS pathogens · 2017Article
- The Lymphocytic Choriomeningitis Virus Matrix Protein PPXY Late Domain Drives the Production of Defective Interfering Particles.PLoS pathogens · 2016Article
- ALIX Rescues Budding of a Double PTAP/PPEY L-Domain Deletion Mutant of Ebola VP40: A Role for ALIX in Ebola Virus Egress.The Journal of infectious diseases · 2015Article
- Calcium Regulation of Hemorrhagic Fever Virus Budding: Mechanistic Implications for Host-Oriented Therapeutic Intervention.PLoS pathogens · 2015Article
- The multifunctional Ebola virus VP40 matrix protein is a promising therapeutic target.Future virology · 2015Article
- The Ebola virus matrix protein VP40 selectively induces vesiculation from phosphatidylserine-enriched membranes.The Journal of biological chemistry · 2014Article
- Conformational plasticity of the Ebola virus matrix protein.Protein science : a publication of the Protein Society · 2014Review
- Reverse genetics systems as tools for the development of novel therapies against filoviruses.Expert review of anti-infective therapy · 2014Review
- Virus nomenclature below the species level: a standardized nomenclature for filovirus strains and variants rescued from cDNA.Archives of virology · 2014Article
- Host IQGAP1 and Ebola virus VP40 interactions facilitate virus-like particle egress.Journal of virology · 2013Article
- The Ebola virus matrix protein penetrates into the plasma membrane: a key step in viral protein 40 (VP40) oligomerization and viral egress.The Journal of biological chemistry · 2013Article
- Viral membrane scission.Annual review of cell and developmental biology · 2013Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Ebola virus particle formation and budding are mediated by the VP40 protein, which possesses overlapping PTAP and PPXY late domain motifs (7-PTAPPXY-13). These late domain motifs have also been found in the Gag proteins of retroviruses and the matrix proteins of rhabdo- and arenaviruses. While in vitro studies suggest a critical role for late domain motifs in the budding of these viruses, including Ebola virus, it remains unclear as to whether the VP40 late domains play a role in Ebola virus replication. Alteration of both late domain motifs drastically reduced VP40 particle formation in vitro. However, using reverse genetics, we were able to generate recombinant Ebola virus containing mutations in either or both of the late domains. Viruses containing mutations in one or both of their late domain motifs were attenuated by one log unit. Transmission and scanning electron microscopy did not reveal appreciable differences between the mutant and wild-type viruses released from infected cells. These findings indicate that the Ebola VP40 late domain motifs enhance virus replication but are not absolutely required for virus replication in cell culture.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.