Trial reportThe Journal of physiology2005
Short-term exercise training in humans reduces AMPK signalling during prolonged exercise independent of muscle glycogen.
Trial report in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed, 1 synthesis or guideline pooled it, 139 citations in OpenAlex.
- Factors Influencing AMPK Activation During Cycling Exercise: A Pooled Analysis and Meta-Regression.Sports medicine (Auckland, N.Z.) · 2022Pooled it
- Phosphoproteomics Uncovers Exercise Intensity-Specific Skeletal Muscle Signaling Networks Underlying High-Intensity Interval Training in Healthy Male Participants.Sports medicine (Auckland, N.Z.) · 2025Trial
- The Influence of Acute Oral Lactate Supplementation on Responses to Cycle Ergometer Exercise: A Randomized, Crossover Pilot Clinical Trial.Nutrients · 2024Trial
- Frequent interruptions of sedentary time modulates contraction- and insulin-stimulated glucose uptake pathways in muscle: Ancillary analysis from randomized clinical trials.Scientific reports · 2016Trial
- Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes.American journal of physiology. Regulatory, integrative and comparative physiology · 2016Trial
- Role of exercise duration on metabolic adaptations in working muscle to short-term moderate-to-heavy aerobic-based cycle training.European journal of applied physiology · 2013Trial
- Trial
- Training in the fasted state facilitates re-activation of eEF2 activity during recovery from endurance exercise.European journal of applied physiology · 2011Trial
- N-Acetylcysteine infusion does not affect glucose disposal during prolonged moderate-intensity exercise in humans.The Journal of physiology · 2010Trial
- Context-dependent regulation of IL-6 in exercise and colorectal cancer cachexia.Frontiers in oncology · 2026Review
- Review
- Effects of aging and resistance exercise on muscle strength, physiological properties, longevity proteins, and telomere length in SAMP8 mice.Biogerontology · 2025Article
- Mechanical efficiency: associations with body composition and glycemic profile in healthy adults.European journal of applied physiology · 2024Article
- The impact of aerobic and anaerobic exercise interventions on the management and outcomes of non-alcoholic fatty liver disease.Physiological research · 2024Review
- Training Session Models in Endurance Sports: A Norwegian Perspective on Best Practice Recommendations.Sports medicine (Auckland, N.Z.) · 2024Article
- The Effect of Cold-Water Swimming on Energy Metabolism, Dynamics, and Mitochondrial Biogenesis in the Muscles of Aging Rats.International journal of molecular sciences · 2024Article
- The Immediate Effects of Aerobic Exercise with and Without External Loads on Blood Glucose, Cardiovascular, Respiratory, and Body Temperature Indices in Type II Diabetic Patients.The journal of Tehran Heart Center · 2023Article
- Factors Influencing Substrate Oxidation During Submaximal Cycling: A Modelling Analysis.Sports medicine (Auckland, N.Z.) · 2022Article
- Protein signalling in response to ex vivo dynamic contractions is independent of training status in rat skeletal muscle.Experimental physiology · 2022Article
- Electrical stimulated GLUT4 signalling attenuates critical illness-associated muscle wasting.Journal of cachexia, sarcopenia and muscle · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We examined the effect of short-term exercise training on skeletal muscle AMP-activated protein kinase (AMPK) signalling and muscle metabolism during prolonged exercise in humans. Eight sedentary males completed 120 min of cycling at 66 +/- 1% , then exercise trained for 10 days, before repeating the exercise bout at the same absolute workload. Participants rested for 72 h before each trial while ingesting a high carbohydrate diet (HCHO). Exercise training significantly (P < 0.05) attenuated exercise-induced increases in skeletal muscle free AMP: ATP ratio and glucose disposal and increased fat oxidation. Exercise training abolished the 9-fold increase in AMPK alpha2 activity observed during pretraining exercise. Since training increased muscle glycogen content by 93 +/- 12% (P < 0.01), we conducted a second experiment in seven sedentary male participants where muscle glycogen content was essentially matched pre- and post-training by exercise and a low CHO diet (LCHO; post-training muscle glycogen 52 +/- 7% less than in HCHO, P < 0.001). Despite the difference in muscle glycogen levels in the two studies we obtained very similar results. In both studies the increase in ACCbeta Ser(221) phosphorylation was reduced during exercise after training. In conclusion, there is little activation of AMPK signalling during prolonged exercise following short-term exercise training suggesting that other factors are important in the regulation of glucose disposal and fat oxidation under these circumstances. It appears that muscle glycogen is not an important regulator of AMPK activation during exercise in humans when exercise is begun with normal or high muscle glycogen levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.