ArticleVirology2005
Mutagenesis of the murine hepatitis virus nsp1-coding region identifies residues important for protein processing, viral RNA synthesis, and viral replication.
Article in Virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- A Novel Toolkit of SARS-CoV-2 Sub-Genomic Replicons for Efficient Antiviral Screening.Viruses · 2025Article
- Coronavirus takeover of host cell translation and intracellular antiviral response: a molecular perspective.The EMBO journal · 2024Review
- Identification of intrinsically disorder regions in non-structural proteins of SARS-CoV-2: New insights into drug and vaccine resistance.Molecular and cellular biochemistry · 2022Article
- Genome-Wide Analysis of the Indispensable Role of Non-structural Proteins in the Replication of SARS-CoV-2.Frontiers in microbiology · 2022Article
- A Novel Potentially Recombinant Rodent Coronavirus with a Polybasic Cleavage Site in the Spike Protein.Journal of virology · 2021Article
- The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expression.Cell reports · 2021Article
- Structure of Nonstructural Protein 1 from SARS-CoV-2.Journal of virology · 2021Article
- Article
- Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy.Nucleic acids research · 2020Article
- SARS-CoV-2 Disrupts Splicing, Translation, and Protein Trafficking to Suppress Host Defenses.Cell · 2020Article
- Structure of nonstructural protein 1 from SARS-CoV-2.bioRxiv : the preprint server for biology · 2020Article
- Emerging of a SARS-CoV-2 viral strain with a deletion in nsp1.Journal of translational medicine · 2020Article
- Computational Inference of Selection Underlying the Evolution of the Novel Coronavirus, Severe Acute Respiratory Syndrome Coronavirus 2.Journal of virology · 2020Article
- MERS coronavirus nsp1 participates in an efficient propagation through a specific interaction with viral RNA.Virology · 2017Article
- Coronavirus cis-Acting RNA Elements.Advances in virus research · 2016Review
- Article
- The structure and functions of coronavirus genomic 3' and 5' ends.Virus research · 2015Review
- Dissection of amino-terminal functional domains of murine coronavirus nonstructural protein 3.Journal of virology · 2015Article
- Coronavirus nonstructural protein 1: Common and distinct functions in the regulation of host and viral gene expression.Virus research · 2015Review
- SHAPE analysis of the RNA secondary structure of the Mouse Hepatitis Virus 5' untranslated region and N-terminal nsp1 coding sequences.Virology · 2015Article
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2 authors.
Funding
Abstract
Despite ongoing research investigating mechanisms of coronavirus replication, functions of many viral nonstructural proteins (nsps) remain unknown. In the current study, a reverse genetic approach was used to define the role of the 28-kDa amino-terminal product (nsp1) of the gene 1 polyprotein during replication of the coronavirus murine hepatitis virus (MHV) in cell culture. To determine whether nsp1 is required for MHV replication and to identify residues critical for protein function, mutant viruses that contained deletions or point mutations within the nsp1-coding region were generated and assayed for defects in viral replication, viral protein expression, protein localization, and RNA synthesis. The results demonstrated that the carboxy-terminal half of nsp1 (residues K(124) through L(241)) was dispensable for virus replication in culture but was required for efficient proteolytic cleavage of nsp1 from the gene 1 polyprotein and for optimal viral replication. Furthermore, whereas deletion of nsp1 residues amino-terminal to K(124) failed to produce infectious virus, point mutagenesis of the nsp1 amino-terminus allowed recovery of several mutants with altered replication and RNA synthesis. This study identifies nsp1 residues important for protein processing, viral RNA synthesis, and viral replication.
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