ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2005
Construction and characterization of a peanut HindIII BAC library.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 40 citations in OpenAlex.
- A genome-wide BAC-end sequence survey provides first insights into sweetpotato (Ipomoea batatas (L.) Lam.) genome composition.BMC genomics · 2016Article
- Construction of a high-quality genomic BAC library for Chinese peanut cultivar Zhonghua 8 with high oil content.Botanical studies · 2014Article
- Comparative and evolutionary analysis of major peanut allergen gene families.Genome biology and evolution · 2014Article
- The repetitive component of the A genome of peanut (Arachis hypogaea) and its role in remodelling intergenic sequence space since its evolutionary divergence from the B genome.Annals of botany · 2013Article
- Characterization and compilation of polymorphic simple sequence repeat (SSR) markers of peanut from public database.BMC research notes · 2012Article
- Characterization of active miniature inverted-repeat transposable elements in the peanut genome.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Matita, a new retroelement from peanut: characterization and evolutionary context in the light of the Arachis A-B genome divergence.Molecular genetics and genomics : MGG · 2012Article
- Advances in BAC-based physical mapping and map integration strategies in plants.Journal of biomedicine & biotechnology · 2012Review
- BAC library resources for map-based cloning and physical map construction in barley (Hordeum vulgare L.).BMC genomics · 2011Article
- Construction, characterization, and preliminary BAC-end sequence analysis of a bacterial artificial chromosome library of the tea plant (Camellia sinensis).Journal of biomedicine & biotechnology · 2011Article
- A BAC library of Beta vulgaris L. for the targeted isolation of centromeric DNA and molecular cytogenetics of Beta species.Genetica · 2009Article
- BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut.BMC plant biology · 2008Article
- Construction of BAC and BIBAC libraries from sunflower and identification of linkage group-specific clones by overgo hybridization.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial artificial chromosome (BAC) libraries have been an essential tool for physical analyses of genomes of many crops. We constructed and characterized the first large-insert DNA library for Arachis hypogaea L. The HindIII BAC library contains 182,784 clones; only 5,484 (3%) had no inserts; and the average insert size is 104.05 kb. Chloroplast DNA contamination was very low, only nine clones, and r-DNA content was 1,208, 0.66% of clones. The depth of coverage is estimated to be 6.5 genome-equivalents, allowing the isolation of virtually any single-copy locus. This rate of coverage was confirmed with the application of 20 overgos, which identified 305 positive clones from the library. The identification of multiple loci by most probes in polyploids complicates anchoring of physical and genetic maps. We explored the practicality of a hybridization-based approach for determination of map locations of BAC clones in peanut by analyzing 94 clones detected by seven different overgos. The banding patterns on Southern blots were good predictors of contig composition; that is, the clones that shared the same size bands and ascribed to the same overgos usually also located in the same contigs. This BAC library has great potential to advance future research about the peanut genome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.