ArticleAntimicrobial agents and chemotherapy2005
Secretion of proteases by Pseudomonas aeruginosa biofilms exposed to ciprofloxacin.
Article in Antimicrobial agents and chemotherapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 92 citations in OpenAlex.
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- Spondias pinnata mediated silver nanoparticles with antibiofilm and catalytic potential.Scientific reports · 2025Article
- Gelatin-Based Microspheres of Ciprofloxacin for Enhanced Lung Delivery and Biofilm Eradication inGels (Basel, Switzerland) · 2025Article
- Modulation of pulmonary immune functions by theFrontiers in immunology · 2025Review
- Theophylline as a quorum sensing and biofilm inhibitor in Pseudomonas aeruginosa and Chromobacterium violaceum.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Prevalence and Virulence of Commensal Pseudomonas Aeruginosa Isolates from Healthy Individuals in Southern Vietnam (2018-2020).Biomedicines · 2022Article
- Comparative metabolite profiling of four polyphenol rich Morus leaves extracts in relation to their antibiofilm activity against Enterococcus faecalis.Scientific reports · 2022Article
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- 6-Methylcoumarin attenuates quorum sensing and biofilm formation in Pseudomonas aeruginosa PAO1 and its applications on solid surface coatings with polyurethane.Applied microbiology and biotechnology · 2021Article
- The Role of Elastase in Corneal Epithelial Barrier Dysfunction Caused by Pseudomonas aeruginosa Exoproteins.Investigative ophthalmology & visual science · 2021Article
- Lipoxin A4 promotes reduction and antibiotic efficacy against Pseudomonas aeruginosa biofilm.Prostaglandins & other lipid mediators · 2021Article
- Coordination of las regulated virulence factors with Multidrug-Resistant and extensively drug-resistant in superbug strains of P. aeruginosa.Molecular biology reports · 2020Article
- Anaerobiosis influences virulence properties of Pseudomonas aeruginosa cystic fibrosis isolates and the interaction with Staphylococcus aureus.Scientific reports · 2019Article
- Fucoidan-Stabilized Gold Nanoparticle-Mediated Biofilm Inhibition, Attenuation of Virulence and Motility Properties inMarine drugs · 2019Article
- Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome.Microbiome · 2019Article
- Antimicrobial susceptibility and virulence genes of clinical and environmental isolates ofPeerJ · 2019Article
- Ciprofloxacin binding to GyrA causes global changes in the proteome of Pseudomonas aeruginosa.FEMS microbiology letters · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas aeruginosa proteases are considered important virulence factors which damage host tissues and interfere with host antibacterial defense mechanisms. P. aeruginosa biofilm cells are not completely killed by antibacterials, and therefore this study addresses the question whether ciprofloxacin attenuates the virulence of biofilm communities by abolishing their secretion of proteases. The surviving cells of the colony biofilms studied, despite their cyclical exposure to four doses of ciprofloxacin at bactericidal concentrations (one dose a day), still secreted active proteases to the environment surrounding the biofilms. The biofilm cells secreted elastase B (LasB) over the duration of the experiments as confirmed by Western immunoblot analysis. The colony biofilms did not secrete LasA-a protease with staphylolytic activity. The same profiles on zymogram gels with gelatin were observed for the proteases secreted by both ciprofloxacin-exposed and unexposed (control) biofilms. Total proteolytic activities of the colony biofilms studied were significantly reduced after exposure to ciprofloxacin at bactericidal concentrations-after 96 h of exposure they dropped to 38% for the strain intermediate resistant to ciprofloxacin and to 65% for the strain highly resistant to the antibiotic, relative to the control biofilms. The surviving cells of the colony biofilms after their release into a fresh medium displayed transient increased resistance to ciprofloxacin compared to their planktonic counterparts.
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