ArticleAntimicrobial agents and chemotherapy2005
Production and characterization of stable amphotericin-resistant amastigotes and promastigotes of Leishmania mexicana.
Article in Antimicrobial agents and chemotherapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Microneedle-based injection of Fungizone/Amphotericin B: an effective treatment for American cutaneous leishmaniasis in mice.Drug delivery · 2026Article
- Amphotericin B resistance correlates with increased fitnessFrontiers in microbiology · 2023Article
- Amphotericin B resistance in Leishmania mexicana: Alterations to sterol metabolism and oxidative stress response.PLoS neglected tropical diseases · 2022Article
- Impact of Genetic Diversity and Genome Plasticity ofFrontiers in cellular and infection microbiology · 2022Review
- LmjF.22.0810 fromBiomedicines · 2020Article
- Effectiveness and Safety of Amphotericin B Deoxycholate, Amphotericin B Colloidal Dispersion, and Liposomal Amphotericin B as Third-Line Treatments for Cutaneous and Mucocutaneous Leishmaniasis: A Retrospective Study.The American journal of tropical medicine and hygiene · 2020Article
- Antileishmanial Activity and Synergistic Effects of Amphotericin B Deoxycholate with Allicin and Andrographolide againstPathogens (Basel, Switzerland) · 2020Article
- Genomic instability at the locus of sterol C24-methyltransferase promotes amphotericin B resistance in Leishmania parasites.PLoS neglected tropical diseases · 2019Article
- Untargeted metabolomics to understand the basis of phenotypic differences in amphotericin B-resistantWellcome open research · 2019Article
- Evaluating drug resistance in visceral leishmaniasis: the challenges.Parasitology · 2018Review
- Intraspecies differences in natural susceptibility to amphotericine B of clinical isolates of Leishmania subgenus Viannia.PloS one · 2018Article
- Drug resistance and treatment failure in leishmaniasis: A 21st century challenge.PLoS neglected tropical diseases · 2017Review
- Sterol 14α-demethylase mutation leads to amphotericin B resistance in Leishmania mexicana.PLoS neglected tropical diseases · 2017Article
- Comparative Fitness of a Parent Leishmania donovani Clinical Isolate and Its Experimentally Derived Paromomycin-Resistant Strain.PloS one · 2015Article
- Fitness and Phenotypic Characterization of Miltefosine-Resistant Leishmania major.PLoS neglected tropical diseases · 2015Article
- Efficacy of intralesional amphotericin B for the treatment of cutaneous leishmaniasis.Indian journal of dermatology · 2014Article
- Comparative analysis of the omics technologies used to study antimonial, amphotericin B, and pentamidine resistance in leishmania.Journal of parasitology research · 2014Review
- Anti-leishmanial effects of purified compounds from aerial parts of Baccharis uncinella C. DC. (Asteraceae).Parasitology research · 2011Article
- Efficacy of Topical Liposomal Amphotericin B versus Intralesional Meglumine Antimoniate (Glucantime) in the Treatment of Cutaneous Leishmaniasis.Journal of parasitology research · 2011Article
- Selection and phenotype characterization of potassium antimony tartrate-resistant populations of four New World Leishmania species.Parasitology research · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sensitivities of Leishmania mexicana amastigote and promastigote forms to amphotericin B were investigated in vitro and found to be strongly influenced by the culture media used. When differences in culture media were minimized, there was no significant difference in the 50% inhibitory concentration values between the two life cycle stages. Stable amphotericin B-resistant amastigote and promastigote lines were produced by the application of increasing drug pressure to long-term cultures. Lines capable of growth in concentrations of amphotericin B lethal to normal parasites were produced. Compared to normal parasites, these amphotericin-resistant lines showed marked differences in membrane sterol compositions, with very high levels of 4,14,dimethyl-cholesta-8,24-dienol and other methyl sterols. They also showed a consistent morphological feature, the presence of multilamellar membrane-like material in the flagellar pocket, revealed by transmission electron microscopy. Amphotericin-resistant parasites were capable of infecting BALB/c mice, but the resulting lesion growth was slower than that after infection with normal parasites. However, unlike normal parasites, the amphotericin-resistant parasites were unaffected by experimental chemotherapy with amphotericin B. These results show that amphotericin B resistance could arise as a result of increased clinical use of amphotericin B therapy.
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