ArticleMolecular microbiology2005
Disruption of the developmental programme of Trypanosoma brucei by genetic ablation of TbZFP1, a differentiation-enriched CCCH protein.
Article in Molecular microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Trypanosoma cruzi cell atlas as a single-cell resource for understanding parasite population heterogeneity and differentiation.Nature communications · 2026Article
- Functional Characterization of Eight Zinc Finger Motif-Containing Proteins inPathogens (Basel, Switzerland) · 2023Article
- Article
- Role of the RNA-binding protein ZC3H41 in the regulation of ribosomal protein messenger RNAs in trypanosomes.Parasites & vectors · 2023Article
- Interactions of theParasitology · 2022Article
- Nuclear mRNA maturation and mRNA export control: from trypanosomes to opisthokonts.Parasitology · 2021Review
- Regulation of Translation in the Protozoan ParasiteInternational journal of molecular sciences · 2020Review
- Translational repression by an RNA-binding protein promotes differentiation to infective forms in Trypanosoma cruzi.PLoS pathogens · 2018Article
- Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection.PLoS pathogens · 2017Article
- RNA-binding proteins related to stress response and differentiation in protozoa.World journal of biological chemistry · 2016Review
- Regulation of RNA binding proteins in trypanosomatid protozoan parasites.World journal of biological chemistry · 2016Review
- The emerging role of RNA-binding proteins in the life cycle of Trypanosoma brucei.Cellular microbiology · 2014Review
- The mRNAs associated to a zinc finger protein from Trypanosoma cruzi shift during stress conditions.RNA biology · 2014Article
- Post-transcriptional regulation of the trypanosome heat shock response by a zinc finger protein.PLoS pathogens · 2013Article
- Bloodstream form pre-adaptation to the tsetse fly in Trypanosoma brucei.Frontiers in cellular and infection microbiology · 2013Review
- The regulation of trypanosome gene expression by RNA-binding proteins.PLoS pathogens · 2013Review
- The post-transcriptional trans-acting regulator, TbZFP3, co-ordinates transmission-stage enriched mRNAs in Trypanosoma brucei.Nucleic acids research · 2012Article
- A zinc finger protein, TbZC3H20, stabilizes two developmentally regulated mRNAs in trypanosomes.The Journal of biological chemistry · 2011Article
- Trans-acting proteins regulating mRNA maturation, stability and translation in trypanosomatids.Trends in parasitology · 2011Review
- Genome-wide in silico screen for CCCH-type zinc finger proteins of Trypanosoma brucei, Trypanosoma cruzi and Leishmania major.BMC genomics · 2010Article
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Authors and funding
2 authors.
Funding
Abstract
The regulation of differentiation is particularly important in microbial eukaryotes that inhabit multiple environments. The parasite Trypanosoma brucei is an extreme example of this, requiring exquisite gene regulation during transmission from mammals to the tsetse fly vector. Unusually, trypanosomes rely almost exclusively on post-transcriptional mechanisms for regulated gene expression. Hence, RNA binding proteins are potentially of great significance in controlling stage-regulated processes. We have previously identified TbZFP1 as a trypanosome molecule transiently enriched during differentiation to tsetse midgut procyclic forms. This small protein (101 amino acids) contains the unusual CCCH zinc finger, an RNA binding motif. Here, we show that genetic ablation of TbZFP1 compromises repositioning of the mitochondrial genome, a specific event in the strictly regulated differentiation programme. Despite this, other events that occur both before and after this remain intact. Significantly, this phenotype correlates with the TbZFP1 expression profile during differentiation. This is the first genetic disruption of a developmental regulator in T. brucei. It demonstrates that programmed events in parasite development can be uncoupled at the molecular level. It also further supports the importance of CCCH proteins in key aspects of trypanosome cell function.
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