ArticleThe Journal of general physiology2005
Probing the geometry of the inner vestibule of BK channels with sugars.
Article in The Journal of general physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 47 citations in OpenAlex.
- Molecular Insights into Single-Chain Lipid Modulation of Acid-Sensing Ion Channel 3.The journal of physical chemistry. B · 2024Article
- Sweetening K-channels: what sugar taught us about permeation and gating.Frontiers in molecular biosciences · 2023Review
- Using Metadynamics To Explore the Free Energy of Dewetting in Biologically Relevant Nanopores.The journal of physical chemistry. B · 2022Article
- Calcium-Activated KHandbook of experimental pharmacology · 2021Article
- Article
- Diffusion Limitations and Translocation Barriers in Atomically Thin Biomimetic Pores.Entropy (Basel, Switzerland) · 2020Article
- The functionally relevant site for paxilline inhibition of BK channels.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Threading the biophysics of mammalian Slo1 channels onto structures of an invertebrate Slo1 channel.The Journal of general physiology · 2017Review
- Pore size matters for potassium channel conductance.The Journal of general physiology · 2016Review
- Effective pore size and radius of capture for K(+) ions in K-channels.Scientific reports · 2016Article
- Pore dimensions and the role of occupancy in unitary conductance of Shaker K channels.The Journal of general physiology · 2015Article
- What keeps Kv channels small? The molecular physiology of modesty.The Journal of general physiology · 2015Article
- Hydrophobic interaction between contiguous residues in the S6 transmembrane segment acts as a stimuli integration node in the BK channel.The Journal of general physiology · 2015Article
- BK channels: multiple sensors, one activation gate.Frontiers in physiology · 2015Review
- Paxilline inhibits BK channels by an almost exclusively closed-channel block mechanism.The Journal of general physiology · 2014Article
- Keeping you healthy: BK channel activation by omega-3 fatty acids.The Journal of general physiology · 2013Article
- Lack of negative slope in I-V plots for BK channels at positive potentials in the absence of intracellular blockers.The Journal of general physiology · 2013Article
- K⁺ conduction and Mg²⁺ blockade in a shaker Kv-channel single point mutant with an unusually high conductance.Biophysical journal · 2012Article
- Stereospecific binding of a disordered peptide segment mediates BK channel inactivation.Nature · 2012Article
- Charge substitution for a deep-pore residue reveals structural dynamics during BK channel gating.The Journal of general physiology · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The geometry of the inner vestibule of BK channels was probed by examining the effects of different sugars in the intracellular solution on single-channel current amplitude (unitary current). Glycerol, glucose, and sucrose decreased unitary current through BK channels in a concentration- and size-dependent manner, in the order sucrose > glucose > glycerol, with outward currents being reduced more than inward currents. The fractional decrease of outward current was more directly related to the fractional hydrodynamic volume occupied by the sugars than to changes in osmolality. For concentrations of sugars < or =1 M, the i/V plots for outward currents in the presence and absence of sugar superimposed after scaling, and increasing K(+)(i) from 150 mM to 2 M increased the magnitudes of the i/V plots with little effect on the shape of the scaled curves. These observations suggest that sugars < or =1 M reduce outward currents mainly by entering the inner vestibule and reducing the movement of K(+) through the vestibule, rather than by limiting diffusion-controlled access of K(+) to the vestibule. With 2 M sucrose, the movement of K(+) into the inner vestibule became diffusion limited for 150 mM K(+)(i) and voltages > +100 mV. Increasing K(+)(i) then relieved the diffusion limitation. An estimate of the capture radius based on the 5 pA diffusion-limited current for channels without the ring of negative charge at the entrance to the inner vestibule was 2.2 A. Adding the radius of a hydrated K(+) (6-8 A) then gave an effective radius for the entrance to the inner vestibule of 8-10 A. Such a functionally wide entrance to the inner vestibule together with our observation that even small concentrations of sugar in the inner vestibule reduce unitary current suggest that a wide inner vestibule is required for the large conductance of BK channels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.