ArticleProceedings of the National Academy of Sciences of the United States of America2005
Electrically monitoring DNA repair by photolyase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 57 citations in OpenAlex.
- A Robust Equivalent Circuit Model for DNA-Mediated Electrochemistry.ChemPlusChem · 2026Article
- Molecular wrench activity of DNA helicases: Keys to modulation of rapid kinetics in DNA repair.Protein science : a publication of the Protein Society · 2023Article
- Following anticancer drug activity in cell lysates with DNA devices.Biosensors & bioelectronics · 2018Article
- Fast and simple method for screening of single-stranded DNA breaking photosensitizers using graphene oxide.Nano convergence · 2018Article
- Sensing DNA through DNA Charge Transport.ACS chemical biology · 2018Review
- A Compass at Weak Magnetic Fields Using Thymine Dimer Repair.ACS central science · 2018Article
- Electrical Probes of DNA-Binding Proteins.Methods in enzymology · 2017Article
- Examining cooperative binding of Sox2 on DC5 regulatory element upon complex formation with Pax6 through excess electron transfer assay.Nucleic acids research · 2016Article
- DNA charge transport within the cell.Biochemistry · 2015Review
- Monitoring cooperative binding using electrochemical DNA-based sensors.Langmuir : the ACS journal of surfaces and colloids · 2015Article
- Solution, surface, and single molecule platforms for the study of DNA-mediated charge transport.Physical chemistry chemical physics : PCCP · 2012Review
- Density functional theory studies of the extent of hole delocalization in one-electron oxidized adenine and guanine base stacks.The journal of physical chemistry. B · 2011Article
- Proton-coupled electron transfer in DNA on formation of radiation-produced ion radicals.Chemical reviews · 2010Review
- Searching for a photocycle of the cryptochrome photoreceptors.Current opinion in plant biology · 2010Review
- Multiplexed DNA-modified electrodes.Journal of the American Chemical Society · 2010Article
- DNA-mediated charge transport in redox sensing and signaling.Journal of the American Chemical Society · 2010Article
- First principles effective electronic couplings for hole transfer in natural and size-expanded DNA.The journal of physical chemistry. B · 2009Article
- Scanning electrochemical microscopy of DNA monolayers modified with Nile Blue.Langmuir : the ACS journal of surfaces and colloids · 2008Article
- DNA-mediated electrochemistry.Bioconjugate chemistry · 2008Review
- Biological contexts for DNA charge transport chemistry.Current opinion in chemical biology · 2008Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cyclobutane pyrimidine dimers are the major DNA photoproducts produced upon exposure to UV radiation. If left unrepaired, these lesions can lead to replication errors, mutation, and cell death. Photolyase is a light-activated flavoenzyme that binds to pyrimidine dimers in DNA and repairs them in a reaction triggered by electron transfer from the photoexcited flavin cofactor to the dimer. Using gold electrodes modified with DNA duplexes containing a cyclobutane thymine dimer (T<>T), here we probe the electrochemistry of the flavin cofactor in Escherichia coli photolyase. Cyclic and square-wave voltammograms of photolyase deposited on these electrodes show a redox signal at 40 mV versus normal hydrogen electrode, consistent with electron transfer to and from the flavin in the DNA-bound protein. This signal is dramatically attenuated on surfaces where the pi-stacking of the DNA bases is perturbed by the presence of an abasic site below the T<>T, an indication that the redox pathway is DNA-mediated. DNA repair can, moreover, be monitored electrically. Exposure of photolyase on T<>T-damaged DNA films to near-UV/blue light leads to changes in the flavin signal consistent with repair, as confirmed by parallel HPLC experiments. These results demonstrate the exquisite sensitivity of DNA electrochemistry to perturbations in base pair stacking and the applicability of this chemistry to probe reactions of proteins with DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.