ArticleRNA (New York, N.Y.)2005
The role of ribosome recycling factor in dissociation of 70S ribosomes into subunits.
Article in RNA (New York, N.Y.), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 94 citations in OpenAlex.
- Role of ribosome recycling factor in natural termination and translational coupling as a ribosome releasing factor.PloS one · 2023Article
- Distinct mechanisms of the human mitoribosome recycling and antibiotic resistance.Nature communications · 2021Article
- Specialized Metabolites from Ribosome Engineered Strains ofMetabolites · 2021Article
- Comparative Analysis of Structural and Dynamical Features of Ribosome Upon Association With mRNA Reveals Potential Role of Ribosomal Proteins.Frontiers in molecular biosciences · 2021Article
- Enhanced Symbiotic Characteristics in Bacterial Genomes with the Disruption of rRNA Operon.Biology · 2020Article
- Article
- Dissecting macromolecular recognition sites in ribosome: implication to its self-assembly.RNA biology · 2019Article
- Pneumococcal RNase R globally impacts protein synthesis by regulating the amount of actively translating ribosomes.RNA biology · 2019Article
- Effects of polyamines on protein synthesis and growth ofThe Journal of biological chemistry · 2018Review
- The kinetic mechanism of bacterial ribosome recycling.Nucleic acids research · 2017Article
- Disassembly of theProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Post-termination Ribosome Intermediate Acts as the Gateway to Ribosome Recycling.Cell reports · 2017Article
- A Possible Role of the Full-Length Nascent Protein in Post-Translational Ribosome Recycling.PloS one · 2017Article
- PdtaS Deficiency Affects Resistance of Mycobacteria to Ribosome Targeting Antibiotics.Frontiers in microbiology · 2017Article
- Article
- Mechanism of fusidic acid inhibition of RRF- and EF-G-dependent splitting of the bacterial post-termination ribosome.Nucleic acids research · 2016Article
- 70S-scanning initiation is a novel and frequent initiation mode of ribosomal translation in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- Complete kinetic mechanism for recycling of the bacterial ribosome.RNA (New York, N.Y.) · 2016Article
- New insights into the enzymatic role of EF-G in ribosome recycling.Nucleic acids research · 2015Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Protein synthesis is initiated on ribosomal subunits. However, it is not known how 70S ribosomes are dissociated into small and large subunits. Here we show that 70S ribosomes, as well as the model post-termination complexes, are dissociated into stable subunits by cooperative action of three translation factors: ribosome recycling factor (RRF), elongation factor G (EF-G), and initiation factor 3 (IF3). The subunit dissociation is stable enough to be detected by conventional sucrose density gradient centrifugation (SDGC). GTP, but not nonhydrolyzable GTP analog, is essential in this process. We found that RRF and EF-G alone transiently dissociate 70S ribosomes. However, the transient dissociation cannot be detected by SDGC. IF3 stabilizes the dissociation by binding to the transiently formed 30S subunits, preventing re-association back to 70S ribosomes. The three-factor-dependent stable dissociation of ribosomes into subunits completes the ribosome cycle and the resulting subunits are ready for the next round of translation.
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