ArticleRNA (New York, N.Y.)2005
A mutational analysis of U12-dependent splice site dinucleotides.
Article in RNA (New York, N.Y.), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Where the minor things are: a pan-eukaryotic survey suggests neutral processes may explain much of minor intron evolution.Nucleic acids research · 2023Article
- Intronic position +9 and -9 are potentially splicing sites boundary from intronic variants analysis of whole exome sequencing data.BMC medical genomics · 2023Article
- Distinct regulatory functions and biological roles of lncRNA splice variants.Molecular therapy. Nucleic acids · 2023Review
- Chromosomal instability by mutations in the novel minor spliceosome component CENATAC.The EMBO journal · 2021Article
- Comprehensive database and evolutionary dynamics of U12-type introns.Nucleic acids research · 2020Article
- New insights into minor splicing-a transcriptomic analysis of cells derived from TALS patients.RNA (New York, N.Y.) · 2019Article
- A comprehensive survey of non-canonical splice sites in the human transcriptome.Nucleic acids research · 2014Article
- The U2AF35-related protein Urp contacts the 3' splice site to promote U12-type intron splicing and the second step of U2-type intron splicing.Genes & development · 2010Article
- Evolutionary dynamics of U12-type spliceosomal introns.BMC evolutionary biology · 2010Article
- The conserved 3' end domain of U6atac snRNA can direct U6 snRNA to the minor spliceosome.RNA (New York, N.Y.) · 2009Article
- Using profiles based on nucleotide hydrophobicity to define essential regions for splicing.International journal of biological sciences · 2009Article
- Mutational analysis of the U12-dependent branch site consensus sequence.RNA (New York, N.Y.) · 2008Article
- The U11-48K protein contacts the 5' splice site of U12-type introns and the U11-59K protein.Molecular and cellular biology · 2008Article
- A comprehensive computational characterization of conserved mammalian intronic sequences reveals conserved motifs associated with constitutive and alternative splicing.Genome research · 2007Article
- U12DB: a database of orthologous U12-type spliceosomal introns.Nucleic acids research · 2007Article
- Alternative splicing and bioinformatic analysis of human U12-type introns.Nucleic acids research · 2007Article
- Aberrant 3' splice sites in human disease genes: mutation pattern, nucleotide structure and comparison of computational tools that predict their utilization.Nucleic acids research · 2006Article
- Comprehensive splice-site analysis using comparative genomics.Nucleic acids research · 2006Article
- The significant other: splicing by the minor spliceosome.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Introns spliced by the U12-dependent minor spliceosome are divided into two classes based on their splice site dinucleotides. The /AU-AC/ class accounts for about one-third of U12-dependent introns in humans, while the /GU-AG/ class accounts for the other two-thirds. We have investigated the in vivo and in vitro splicing phenotypes of mutations in these dinucleotide sequences. A 5' A residue can splice to any 3' residue, although C is preferred. A 5' G residue can splice to 3' G or U residues with a preference for G. Little or no splicing was observed to 3' A or C residues. A 5' U or C residue is highly deleterious for U12-dependent splicing, although some combinations, notably 5' U to 3' U produced detectable spliced products. The dependence of 3' splice site activity on the identity of the 5' residue provides evidence for communication between the first and last nucleotides of the intron. Most mutants in the second position of the 5' splice site and the next to last position of the 3' splice site were defective for splicing. Double mutants of these residues showed no evidence of communication between these nucleotides. Varying the distance between the branch site and the 3' splice site dinucleotide in the /GU-AG/ class showed that a somewhat larger range of distances was functional than for the /AU-AC/ class. The optimum branch site to 3' splice site distance of 11-12 nucleotides appears to be the same for both classes.
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Registered trials
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