ArticleBiochemical and biophysical research communications2005
Binding of two zinc finger nuclease monomers to two specific sites is required for effective double-strand DNA cleavage.
Article in Biochemical and biophysical research communications, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 92 citations in OpenAlex.
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- Non-Viral Delivery To Enable Genome Editing.Trends in biotechnology · 2019Review
- Genome editing for the treatment of tumorigenic viral infections and virus-related carcinomas.Frontiers of medicine · 2018Review
- Genome Editing Tools in Plants.Genes · 2017Review
- Gene Editing and Human Pluripotent Stem Cells: Tools for Advancing Diabetes Disease Modeling and Beta-Cell Development.Current diabetes reports · 2017Review
- A history of genome editing in mammals.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- Review
- A novel arrangement of zinc finger nuclease system for in vivo targeted genome engineering: the tomato LEC1-LIKE4 gene case.Plant cell reports · 2016Article
- Origins of Programmable Nucleases for Genome Engineering.Journal of molecular biology · 2016Review
- Recent developments and clinical studies utilizing engineered zinc finger nuclease technology.Cellular and molecular life sciences : CMLS · 2015Review
- Chapter 3 - Restoring Vision to the Blind: Gene Therapy for Vision Loss.Translational vision science & technology · 2014Article
- Epstein-Barr virus BALF3 has nuclease activity and mediates mature virion production during the lytic cycle.Journal of virology · 2014Article
- Generation of mastitis resistance in cows by targeting human lysozyme gene to β-casein locus using zinc-finger nucleases.Proceedings. Biological sciences · 2014Article
- Identification of "safe harbor" loci in indica rice genome by harnessing the property of zinc-finger nucleases to induce DNA damage and repair.Frontiers in plant science · 2014Article
- High-efficiency and heritable gene targeting in mouse by transcription activator-like effector nucleases.Nucleic acids research · 2013Article
- Oligodeoxynucleotide binding to (CTG) · (CAG) microsatellite repeats inhibits replication fork stalling, hairpin formation, and genome instability.Molecular and cellular biology · 2013Article
- Targeted gene deletion of miRNAs in mice by TALEN system.PloS one · 2013Article
- Efficient targeted gene disruption in Xenopus embryos using engineered transcription activator-like effector nucleases (TALENs).Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Custom-designed zinc finger nucleases (ZFNs) are becoming powerful tools in gene targeting-the process of replacing a gene within a genome by homologous recombination. Here, we have studied the DNA cleavage by one such ZFN, DeltaQNK-FN, in order to gain insight into how ZFNs cleave DNA and how two inverted sites promote double-strand cleavage. DNA cleavage by DeltaQNK-FN is greatly facilitated when two DeltaQNK-binding sites are close together in an inverted orientation. Substrate cleavage was not first order with respect to the concentration of DeltaQNK-FN, indicating that double-strand cleavage requires dimerization of the FokI cleavage domain. Rates of DNA cleavage decrease as the substrate concentrations increase, suggesting that the DeltaQNK-FN molecules are effectively "trapped" in a 1:1 complex on DNA when the DNA is in excess. The physical association of two ZFN monomers on DNA was monitored by using the biotin-pull-down assay, which showed that the formation of DeltaQNK-FN active complex required both binding of the two DeltaQNK-FN molecules to specific DNA sites and divalent metal ions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.