ArticleBMC genomics2005
Conjugative plasmid pAW63 brings new insights into the genesis of the Bacillus anthracis virulence plasmid pXO2 and of the Bacillus thuringiensis plasmid pBT9727.
Article in BMC genomics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Antimicrobial Resistance Profile, Whole-Genome Sequencing and Core Genome Multilocus Sequence Typing ofAntibiotics (Basel, Switzerland) · 2024Article
- Comparative analysis of Listeria monocytogenes plasmid transcriptomes reveals common and plasmid-specific gene expression patterns and high expression of noncoding RNAs.MicrobiologyOpen · 2022Article
- Antimicrobial activity and safety features assessment ofFood science & nutrition · 2022Article
- Conjugation acrossFrontiers in microbiology · 2022Review
- Article
- Rap-Phr Systems from Plasmids pAW63 and pHT8-1 Act Together To Regulate Sporulation in the Bacillus thuringiensis Serovar kurstaki HD73 Strain.Applied and environmental microbiology · 2020Article
- Diversity of Bacillus cereus sensu lato mobilome.BMC genomics · 2019Article
- Regulons and protein-protein interactions of PRD-containing Bacillus anthracis virulence regulators reveal overlapping but distinct functions.Molecular microbiology · 2018Article
- The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen.Molecular genetics, microbiology and virology : Molekulyarnaya genetika, mikrobiologiya i virusologiya · 2017Article
- A Type III protein-RNA toxin-antitoxin system from Bacillus thuringiensis promotes plasmid retention during spore development.RNA biology · 2015Article
- Virulence Plasmids of Spore-Forming Bacteria.Microbiology spectrum · 2014Review
- Novel RNA structural features of an alternatively splicing group II intron from Clostridium tetani.RNA (New York, N.Y.) · 2014Article
- Review
- Selectivity and self-assembly in the control of a bacterial toxin by an antitoxic noncoding RNA pseudoknot.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Cloning and analysis of a large plasmid pBMB165 from Bacillus thuringiensis revealed a novel plasmid organization.PloS one · 2013Article
- Comparative analysis of plasmids in the genus Listeria.PloS one · 2010Article
- Article
- High-throughput genome sequencing of two Listeria monocytogenes clinical isolates during a large foodborne outbreak.BMC genomics · 2010Article
- Conjugative transfer of insecticidal plasmid pHT73 from Bacillus thuringiensis to B. anthracis and compatibility of this plasmid with pXO1 and pXO2.Applied and environmental microbiology · 2010Article
- Bacillus anthracis physiology and genetics.Molecular aspects of medicine · 2009Review
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3 authors.
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Abstract
backgroundBacillus cereus, Bacillus anthracis and Bacillus thuringiensis belong to the genetically close-knit Bacillus cereus sensu lato group, a family of rod-shaped Gram-positive bacteria. pAW63 is the first conjugative plasmid from the B. cereus group to be completely sequenced.
resultsThe 71,777 bp nucleotide sequence of pAW63 reveals a modular structure, including a 42 kb tra region encoding homologs of the Type IV secretion systems components VirB11, VirB4 and VirD4, as well as homologs of Gram-positive conjugation genes from Enterococcus, Lactococcus, Listeria, Streptococcus and Staphylococcus species. It also firmly establishes the existence of a common backbone between pAW63, pXO2 from Bacillus anthracis and pBT9727 from the pathogenic Bacillus thuringiensis serovar konkukian strain 97-27. The alignment of these three plasmids highlights the presence of well conserved segments, in contrast to distinct regions of high sequence plasticity. The study of their specific differences has provided a three-point reference framework that can be exploited to formulate solid hypotheses concerning the functionalities and the molecular evolution of these three closely related plasmids. This has provided insight into the chronology of their divergence, and led to the discovery of two Type II introns on pAW63, matching copies of the mobile element IS231L in different loci of pXO2 and pBT9727, and the identification on pXO2 of a 37 kb pathogenicity island (PAI) containing the anthrax capsule genes.
conclusionThe complete sequence determination of pAW63 has led to a functional map of the plasmid yielding insights into its conjugative apparatus, which includes T4SS-like components, as well as its resemblance to other large plasmids of Gram-positive bacteria. Of particular interest is the extensive homology shared between pAW63 and pXO2, the second virulence plasmid of B. anthracis, as well as pBT9727 from the pathogenic strain B. thuringiensis serovar konkukian strain 97-27.
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