ArticlePharmaceutical research2005
Region-dependent role of the mucous/glycocalyx layers in insulin permeation across rat small intestinal membrane.
Article in Pharmaceutical research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- Non-transdermal microneedles for advanced drug delivery.Advanced drug delivery reviews · 2020Pooled it
- Characterization of Taurocholic Acid Binding With Insulin for Potential Oral Formulation Using Different Methods.Electrophoresis · 2025Article
- Getting Drugs Across Biological Barriers.Advanced materials (Deerfield Beach, Fla.) · 2017Review
- A review on the strategies for oral delivery of proteins and peptides and their clinical perspectives.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2016Review
- Region-Dependent Role of Cell-Penetrating Peptides in Insulin Absorption Across the Rat Small Intestinal Membrane.The AAPS journal · 2015Article
- Microneedles for drug delivery via the gastrointestinal tract.Journal of pharmaceutical sciences · 2015Article
- Nanoparticles for oral delivery: targeted nanoparticles with peptidic ligands for oral protein delivery.Advanced drug delivery reviews · 2013Review
- Noninvasive routes of proteins and peptides drug delivery.Indian journal of pharmaceutical sciences · 2011Article
- Endothelial surface layer degradation by chronic hyaluronidase infusion induces proteinuria in apolipoprotein E-deficient mice.PloS one · 2010Article
- Insulin-loaded nanoparticles based on N-trimethyl chitosan: in vitro (Caco-2 model) and ex vivo (excised rat jejunum, duodenum, and ileum) evaluation of penetration enhancement properties.AAPS PharmSciTech · 2010Article
- Complexation hydrogels for intestinal delivery of interferon beta and calcitonin.Journal of controlled release : official journal of the Controlled Release Society · 2009Article
- Key functions in polymer carriers for intestinal absorption of insulin.International journal of pharmaceutics · 2008Article
- Novel oral insulin delivery systems based on complexation polymer hydrogels: single and multiple administration studies in type 1 and 2 diabetic rats.Journal of controlled release : official journal of the Controlled Release Society · 2006Article
- Novel mucosal insulin delivery systems based on fusogenic liposomes.Pharmaceutical research · 2006Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regional difference in the contribution of the mucous/glycocalyx layers in rat small intestine, as a diffusional or enzymatic barrier, to the absorption of insulin was investigated by in vitro studies. The mucous/glycocalyx layers from the duodenum, the jejunum, and the ileum in rat were successfully removed without damaging membrane integrity, by exposing them to a hyaluronidase solution in situ. In an in vitro transport experiment, the apparent permeability coefficient (P(app)) of insulin for the hyaluronidase-pretreated group was significantly increased compared to the PBS-pretreated (control) group in all small intestinal regions, and the P(app) of insulin in both PBS- and hyaluronidase-pretreated groups increased in the following order: duodenum < jejunum < ileum. On the other hand, irrespective of small intestinal regions, the P(app) of FD-4 and of antipyrine, respectively the passive para- and transcellular permeation marker, exhibited no significant differences between PBS- and hyaluronidase-pretreated group. In addition, a significant amount of insulin was degraded in the mucous/glycocalyx layers compartment removed by hyaluronidase pretreatment, and the degradation activity in the mucous/glycocalyx layers showed regional differences in the following order: duodenum > jejunum > ileum. These findings suggest that, irrespective of small intestinal regions, the mucous/glycocalyx layers contributed to insulin permeation predominantly as an enzymatic barrier, and not as a diffusional barrier. Furthermore, the variation of the enzymatic activities in the mucous/glycocalyx layers and in the brush-border membrane would be one factor that accounts for the regional differences in the transport of insulin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.