ArticleInfection and immunity2005
Comparison of Helicobacter pylori virulence gene expression in vitro and in the Rhesus macaque.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 54 citations in OpenAlex.
- Hesperetin Inhibits Expression of Virulence Factors and Growth ofInternational journal of molecular sciences · 2021Article
- Evodiamine InhibitsInternational journal of molecular sciences · 2021Article
- Contribution of Heptose Metabolites and theFrontiers in immunology · 2021Article
- Precancerous Gastric Lesions withBioMed research international · 2020Article
- Review
- Inhibitory Effects of Menadione onInternational journal of molecular sciences · 2019Article
- Role of Proline in Pathogen and Host Interactions.Antioxidants & redox signaling · 2019Review
- Overexpression of spoT gene in coccoid forms of clinical Helicobacter pylori isolates.Folia microbiologica · 2018Article
- The cag-pathogenicity island encoded CncR1 sRNA oppositely modulates Helicobacter pylori motility and adhesion to host cells.Cellular and molecular life sciences : CMLS · 2016Article
- Transcriptional Profiling of Type II Toxin-Antitoxin Genes ofFrontiers in microbiology · 2016Article
- Piperine treatment suppresses Helicobacter pylori toxin entry in to gastric epithelium and minimizes β-catenin mediated oncogenesis and IL-8 secretion in vitro.American journal of translational research · 2016Article
- Small-molecule inhibitors of the pseudaminic acid biosynthetic pathway: targeting motility as a key bacterial virulence factor.Antimicrobial agents and chemotherapy · 2014Article
- In depth analysis of the Helicobacter pylori cag pathogenicity island transcriptional responses.PloS one · 2014Article
- Predominant mucosal IL-8 mRNA expression in non-cagA Thais is risk for gastric cancer.World journal of gastroenterology · 2013Article
- Mutations to essential orphan response regulator HP1043 of Helicobacter pylori result in growth-stage regulatory defects.Infection and immunity · 2013Article
- Assessment of cagE and babA mRNA expression during morphological conversion of Helicobacter pylori from spiral to coccoid.Current microbiology · 2013Article
- Molecular diversity of class 2 integrons in antibiotic-resistant gram-negative bacteria found in wastewater environments in China.Ecotoxicology (London, England) · 2013Article
- Functional plasticity in the type IV secretion system of Helicobacter pylori.PLoS pathogens · 2013Article
- Serologic host response to Helicobacter pylori and Campylobacter jejuni in socially housed Rhesus macaques (Macaca mulatta).Gut pathogens · 2012Article
- In vivo expression of Helicobacter pylori virulence genes in patients with gastritis, ulcer, and gastric cancer.Infection and immunity · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
We used a quantitative real-time reverse transcriptase PCR assay to measure the transcript abundance of 46 known and putative Helicobacter pylori virulence genes, including 24 genes on the Cag pathogenicity island. The expression profile of H. pylori cells grown in vitro was also compared to expression in vivo after experimental infection of rhesus macaques. Transcript abundance in vitro (mid-log phase) ranged from about 0.004 (feoB and hpaA) to 20 (ureAB, napA, and cag25) copies/cell. Expression of most genes was repressed during the transition from logarithmic- to stationary-phase growth, but several well-characterized H. pylori virulence genes (katA, napA, vacA, and cagA) were induced. Comparison of results in the rhesus macaque with similar data from humans showed a strong correlation (r = 0.89). The relative in vivo expression in the rhesus monkey was highly correlated with in vitro expression during mid-log (r = 0.89)- and stationary (r = 0.88)-phase growth. Transcript abundance was on average three- to fourfold reduced in vivo compared to in vitro during mid-log phase. However, when compared to stationary phase, increased expression in vivo was observed for 6 of 7 genes on a contiguous portion of the pathogenicity island, several of which are thought to encode the H. pylori type IV structural pilus and its accessory proteins. These results suggest the possibility that some genes encoding the H. pylori type IV structural pilus and accessory proteins may form an operon that is induced during growth in vivo.
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Registered trials
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