ArticleBiophysical journal2005
Quantitative multiphoton spectral imaging and its use for measuring resonance energy transfer.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
74 citing papers in PubMed.
- Fluorescence bioimaging of drug nanocarriers based on Förster resonance energy transfer, aggregation-induced emission and aggregation-caused quenching.Acta pharmaceutica Sinica. B · 2026Review
- Optimal inference of molecular interaction dynamics in FRET microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Genetically Encoded Ratiometric pH Sensors for the Measurement of Intra- and Extracellular pH and Internalization Rates.Biosensors · 2022Article
- Pediatric Encephalopathy: Clinical, Biochemical and Cellular Insights into the Role of Gln52 ofCells · 2021Article
- Deep Learning in Biomedical Optics.Lasers in surgery and medicine · 2021Review
- Investigating tunneling nanotubes in ovarian cancer based on two-photon excitation FLIM-FRET.Biomedical optics express · 2021Article
- Enhancmentation of Photo-Thermal Lens of Fluorescence Molecules by Fluorescence Resonance Energy Transfer Mechanism.Journal of fluorescence · 2021Article
- Probing ion channel macromolecular interactions using fluorescence resonance energy transfer.Methods in enzymology · 2021Article
- Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis.Journal of visualized experiments : JoVE · 2020Article
- UNMIX-ME: spectral and lifetime fluorescence unmixing via deep learning.Biomedical optics express · 2020Article
- QuanTI-FRET: a framework for quantitative FRET measurements in living cells.Scientific reports · 2020Article
- In Vitro FRET- and Fluorescence-Based Assays to Study Protein Conformation and Protein-Protein Interactions in Mitosis.Methods in molecular biology (Clifton, N.J.) · 2020Article
- FRET efficiency measurement in a molecular tension probe with a low-cost frequency-domain fluorescence lifetime imaging microscope.Journal of biomedical optics · 2019Article
- Two-Color Spatial Cumulant Analysis Detects Heteromeric Interactions between Membrane Proteins.Biophysical journal · 2019Article
- FRET as a biomolecular research tool - understanding its potential while avoiding pitfalls.Nature methods · 2019Review
- Ab Initio Derivation of the FRET Equations Resolves Old Puzzles and Suggests Measurement Strategies.Biophysical journal · 2019Article
- Spectral imaging of FRET-based sensors reveals sustained cAMP gradients in three spatial dimensions.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Article
- Measurement of intracellular concentration of fluorescently-labeled targets in living cells.PloS one · 2018Article
- SensorFRET: A Standardless Approach to Measuring Pixel-based Spectral Bleed-through and FRET Efficiency using Spectral Imaging.Scientific reports · 2017Article
- Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy.Optica · 2017Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Protein labeling with green fluorescent protein derivatives has become an invaluable tool in cell biology. Protein quantification, however, is difficult when cells express constructs with overlapping fluorescent emissions. Under these conditions, signal separation using emission filters is inherently inefficient. Spectral imaging solves this problem by recording emission spectra directly. Unfortunately, linear unmixing, the algorithm used for quantifying individual fluorophores from emission spectra, fails when resonance energy transfer (RET) is present. We therefore sought to develop an unmixing algorithm that incorporates RET. An equation for spectral emission incorporating RET was derived and an assay based on this formalism, spectral RET (sRET), was developed. Standards with defined RET efficiencies and with known Cerulean/Venus ratios were constructed and used to test sRET. We demonstrate that sRET analysis is a comprehensive, photon-efficient method for imaging RET efficiencies and accurately determines donor and acceptor concentrations in living cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.