ArticlePlant physiology2005
An RNA-dependent RNA polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 219 papers.
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Who cites it
219 citing papers in PubMed, 489 citations in OpenAlex.
- Small RNA mobility and plant virus diseases.Journal of experimental botany · 2026Review
- Review
- Review
- A method for maintaining the release of co-suppression and maximally restoring the RDR6 expression.Plant cell reports · 2025Article
- Verifying Plasmid Constructs via Transient Agrobacterium tumefaciens-Mediated Plant Transformation in Nicotiana benthamiana.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Enhancing CRISPR-Cas-based gene targeting in tomato using a dominant-negativeHorticulture research · 2025Article
- Heritable Tissue-Culture-Free Gene Editing in Nicotiana benthamiana through Viral Delivery of SpCas9 and sgRNA.Plant & cell physiology · 2024Article
- Engineering VIGS Vectors by Modifying Movement Proteins of the 30K Family.Biotechnology journal · 2024Article
- Conservation of molecular responses upon viral infection in the non-vascular plant Marchantia polymorpha.Nature communications · 2024Article
- Comparative analysis of RNA interference and pattern-triggered immunity induced by dsRNA reveals different efficiencies in the antiviral response to potato virus X.Molecular plant pathology · 2024Article
- Deficiency of multiple RNA silencing-associated genes may contribute to the increased susceptibility of Nicotiana benthamiana to viruses.Plant cell reports · 2024Article
- A novel ilarvirus protein CP-RT is expressed via stop codon readthrough and suppresses RDR6-dependent RNA silencing.PLoS pathogens · 2024Article
- Importin α2 participates in RNA interference against bamboo mosaic virus accumulation in Nicotiana benthamiana via NbAGO10a-mediated small RNA clearance.Molecular plant pathology · 2024Article
- Salicylic acid and RNA interference mediate antiviral immunity of plant stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Infection Defects of RNA and DNA Viruses Induced by Antiviral RNA Interference.Microbiology and molecular biology reviews : MMBR · 2023Review
- Seed transmission of raspberry bushy dwarf virus is blocked in Nicotiana benthamiana plants by preventing virus entry into the embryo from the infected embryo sac and endosperm.Archives of virology · 2023Article
- Differential Effects of RNA-Dependent RNA Polymerase 6 (RDR6) Silencing on New and Old World Begomoviruses inViruses · 2023Article
- A beneficial fungal root endophyte triggers systemic RNA silencing and DNA methylation of a host reporter gene.RNA biology · 2023Article
- A contemporary reassessment of the enhanced transient expression system based on the tombusviral silencing suppressor protein P19.The Plant journal : for cell and molecular biology · 2023Article
- An Evolved 5' Untranslated Region of Alfalfa Mosaic Virus Allows the RNA Transport of Movement-Defective Variants.Journal of virology · 2022Article
159 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the functions of RNA silencing in plants is antiviral defense. A hallmark of RNA silencing is spreading of the silenced state through the plant. Little is known about the nature of the systemic silencing signal and the proteins required for its production, transport, and reception in plant tissues. Here, we show that the RNA-dependent RNA polymerase RDR6 in Nicotiana benthamiana is involved in defense against potato virus X at the level of systemic spreading and in exclusion of the virus from the apical growing point. It has no effect on primary replication and cell-to-cell movement of the virus and does not contribute significantly to the formation of virus-derived small interfering (si) RNA in a fully established potato virus X infection. In grafting experiments, the RDR6 homolog was required for the ability of a cell to respond to, but not to produce or translocate, the systemic silencing signal. Taking these findings together, we suggest a model of virus defense in which RDR6 uses incoming silencing signal to generate double-stranded RNA precursors of secondary siRNA. According to this idea, the secondary siRNAs mediate RNA silencing as an immediate response that slows down the systemic spreading of the virus into the growing point and newly emerging leaves.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.