ArticleJournal of photochemistry and photobiology. B, Biology2005
Monitoring photodynamic therapy of localized infections by bioluminescence imaging of genetically engineered bacteria.
Article in Journal of photochemistry and photobiology. B, Biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 1 synthesis or guideline pooled it, 107 citations in OpenAlex.
- Antimicrobial photodynamic therapy in skin wound healing: A systematic review of animal studies.International wound journal · 2020Pooled it
- 2D MoSNano convergence · 2026Article
- Rose Bengal diacetate-mediated antimicrobial photodynamic inactivation: potentiation by potassium iodide and acceleration of wound healing in MRSA-infected diabetic mice.BMC microbiology · 2024Article
- The Role of Porphyrinoid Photosensitizers for Skin Wound Healing.International journal of molecular sciences · 2021Review
- A Novel Dicationic Boron Dipyrromethene-based Photosensitizer for Antimicrobial Photodynamic Therapy against Methicillin-Resistant Staphylococcus aureus.Current medicinal chemistry · 2021Article
- Longitudinal bioluminescent imaging of HIV-1 infection during antiretroviral therapy and treatment interruption in humanized mice.PLoS pathogens · 2019Article
- Tetracyclines: light-activated antibiotics?Future medicinal chemistry · 2019Review
- Amphiphilic tetracationic porphyrins are exceptionally active antimicrobial photosensitizers: In vitro and in vivo studies with the free-base and Pd-chelate.Journal of biophotonics · 2019Article
- Can light-based approaches overcome antimicrobial resistance?Drug development research · 2019Review
- Article
- A Multimodal Imaging Approach EnablesAntimicrobial agents and chemotherapy · 2018Article
- Antimicrobial photodynamic therapy mediated by methylene blue and potassium iodide to treat urinary tract infection in a female rat model.Scientific reports · 2018Article
- In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging.Virulence · 2018Review
- Potentiation of antimicrobial photodynamic inactivation by inorganic salts.Expert review of anti-infective therapy · 2017Review
- Advances in antimicrobial photodynamic inactivation at the nanoscale.Nanophotonics (Berlin, Germany) · 2017Article
- In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging.Journal of visualized experiments : JoVE · 2017Article
- Antimicrobial photodynamic inactivation: a bright new technique to kill resistant microbes.Current opinion in microbiology · 2016Review
- Photodynamic therapy of oral Candida infection in a mouse model.Journal of photochemistry and photobiology. B, Biology · 2016Article
- Antibacterial photodynamic therapy: overview of a promising approach to fight antibiotic-resistant bacterial infections.Journal of clinical and translational research · 2015Review
- Animal models for photodynamic therapy (PDT).Bioscience reports · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The increasing occurrence of multi-antibiotic resistant microbes has led to the search for alternative methods of killing pathogens and treating infections. Photodynamic therapy (PDT) uses the combination of non-toxic dyes and harmless visible light to produce reactive oxygen species that can kill mammalian and microbial cells. Although the photodynamic inactivation of bacteria has been known for over a hundred years, its use to treat infections has not been much developed. This may be partly due to the difficulty of monitoring the effectiveness of PDT in animal models of infection. In order to facilitate this monitoring process, we have developed a procedure that uses bioluminescent genetically engineered bacteria and a light sensitive imaging system to allow real-time visualization of infections. When these bacteria are treated with PDT in vitro, the loss of luminescence parallels the loss of colony-forming ability. We have developed several models of infections in wounds and soft-tissue abscesses in mice that can be followed by bioluminescence imaging. The size and intensity of the infection can be sequentially monitored in a non-invasive fashion in individual mice in real-time. When photosensitizers are introduced into the infected tissue followed by illumination with red light, a light-dose dependent loss of luminescence is seen. If the bacterium is invasive, the loss of luminescence correlates with increased survival of the mice, whilst animals in control groups die of sepsis within five days. Healing of the PDT treated wounds is not impaired and may actually be improved. This approach can allow many animal models of localized infections to be accurately monitored for efficacy of treatment by PDT.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.