ArticleJournal of biotechnology2005
High level expression of surface glycoprotein of rabies virus in tobacco leaves and its immunoprotective activity in mice.
Article in Journal of biotechnology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 105 citations in OpenAlex.
- Tobacco-Based Vaccines, Hopes, and Concerns: A Systematic Review.Molecular biotechnology · 2023Pooled it
- Review
- Article
- Plant-made vaccines against viral diseases in humans and farm animals.Frontiers in plant science · 2023Review
- Emerging trends of edible vaccine therapy for combating human diseases especially COVID-19: Pros, cons, and future challenges.Phytotherapy research : PTR · 2022Review
- Plant-Derived Recombinant Vaccines against Zoonotic Viruses.Life (Basel, Switzerland) · 2022Review
- Plant-made poliovirus vaccines - Safe alternatives for global vaccination.Frontiers in plant science · 2022Review
- Plant-Produced Vaccines: Future Applications in Aquaculture.Frontiers in plant science · 2021Review
- Production of complex viral glycoproteins in plants as vaccine immunogens.Plant biotechnology journal · 2018Review
- Plant-made vaccines and reagents for the One Health initiative.Human vaccines & immunotherapeutics · 2017Review
- Recombinant Plant Engineering for Immunotherapeutic Production.Current molecular biology reports · 2017Review
- Review
- Oral Rabies Vaccine Design for Expression in Plants.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Current Developments and Future Prospects for Plant-Made Biopharmaceuticals Against Rabies.Molecular biotechnology · 2015Review
- Expression of rabies glycoprotein and ricin toxin B chain (RGP-RTB) fusion protein in tomato hairy roots: a step towards oral vaccination for rabies.Molecular biotechnology · 2015Article
- Plant-based vaccines against viruses.Virology journal · 2014Review
- Effect of recombinant Newcastle disease virus transfection on lung adenocarcinoma A549 cellsOncology letters · 2014Article
- A comparative study of lectin affinity based plant N-glycoproteome profiling using tomato fruit as a model.Molecular & cellular proteomics : MCP · 2014Article
- How can plant genetic engineering contribute to cost-effective fish vaccine development for promoting sustainable aquaculture?Plant molecular biology · 2013Review
- Secretion of truncated recombinant rabies virus glycoprotein with preserved antigenic properties using a co-expression system in Hansenula polymorpha.Journal of microbiology (Seoul, Korea) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A synthetic gene coding for the surface glycoprotein (G protein) of rabies virus was strategically designed to achieve high-level expression in transgenic plants. The native signal peptide was replaced by that of the pathogenesis related protein, PR-S of Nicotiana tabacum. An endoplasmic reticulum retention signal was included at C-terminus of the G protein. Tobacco plants were genetically engineered by nuclear transformation. Selected transgenic lines expressed the chimeric G protein at 0.38% of the total soluble leaf protein. Mice immunized intraperitoneally with the G protein purified from tobacco leaf microsomal fraction elicited high level of immune response as compared to the inactivated commercial viral vaccine. The plant-derived G protein induced complete protective immunity in mice against intracerebral lethal challenge with live rabies virus. The results establish that plants can provide a safe and effective production system for the expression of immunoprotective rabies virus surface protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.