ArticlePlanta2005
Evaluation of control transcripts in real-time RT-PCR expression analysis during maritime pine embryogenesis.
Article in Planta, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 54 citations in OpenAlex.
- Selection of reference genes for gene expression analysis in Liriodendron hybrids' somatic embryogenesis and germinative tissues.Scientific reports · 2021Article
- Transcriptomic Time-Series Analyses of Gene Expression Profile During Zygotic Embryo Development inFrontiers in genetics · 2021Article
- Lead-induced oxidative stress and role of antioxidant defense in wheat (Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Small RNA profiling in Pinus pinaster reveals the transcriptome of developing seeds and highlights differences between zygotic and somatic embryos.Scientific reports · 2019Article
- Genome-Wide Analysis of the YABBY Gene Family in Grapevine and Functional Characterization ofFrontiers in plant science · 2019Article
- Comprehensive assembly and analysis of the transcriptome of maritime pine developing embryos.BMC plant biology · 2018Article
- Comparison of Reliable Reference Genes Following Different Hormone Treatments by Various Algorithms for qRT-PCR Analysis ofInternational journal of molecular sciences · 2018Article
- Article
- Comparative Transcriptomic Approaches Exploring Contamination Stress Tolerance in Salix sp. Reveal the Importance for a Metaorganismal de Novo Assembly Approach for Nonmodel Plants.Plant physiology · 2016Article
- Moderate stress responses and specific changes in polyamine metabolism characterize Scots pine somatic embryogenesis.Tree physiology · 2016Article
- Reference Genes for qPCR Analysis in Resin-Tapped Adult Slash Pine As a Tool to Address the Molecular Basis of Commercial Resinosis.Frontiers in plant science · 2016Article
- Selection and Validation of Reference Genes for Quantitative Real-time PCR in Gentiana macrophylla.Frontiers in plant science · 2016Article
- Selection of Reference Genes for Real-Time Quantitative PCR in Pinus massoniana Post Nematode Inoculation.PloS one · 2016Article
- Evaluation of Appropriate Reference Genes for Reverse Transcription-Quantitative PCR Studies in Different Tissues of a Desert Poplar via Comparision of Different Algorithms.International journal of molecular sciences · 2015Article
- Expression of catalase and retinoblastoma-related protein genes associates with cell death processes in Scots pine zygotic embryogenesis.BMC plant biology · 2015Article
- Differential Gene Expression Reveals Candidate Genes for Drought Stress Response in Abies alba (Pinaceae).PloS one · 2015Article
- Quantitative detection of Staphylococcus aureus, Streptococcus pneumoniae and Haemophilus influenzae in patients with new influenza A (H1N1)/2009 and influenza A/2010 virus infection.GMS hygiene and infection control · 2015Article
- Normalizing gene expression by quantitative PCR during somatic embryogenesis in two representative conifer species: Pinus pinaster and Picea abies.Plant cell reports · 2013Article
- Selection and validation of suitable reference genes for miRNA expression normalization by quantitative RT-PCR in citrus somatic embryogenic and adult tissues.Plant cell reports · 2012Article
- Selection and validation of reference genes for quantitative RT-PCR expression studies of the non-model crop Musa.Molecular breeding : new strategies in plant improvement · 2012Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to determine the suitability of reference or housekeeping genes as internal controls in real-time reverse transcriptase PCR (RT-PCR) assays for quantification of target mRNAs, we studied the levels of expression of four candidate reference genes in maritime pine by real-time RT-PCR. The expression levels obtained for glyceraldehyde-3-phosphate-dehydrogenase, 18S ribosomal RNA, eukaryotic translation initiation factor eIF4AII and ubiquitin in nine stages of embryo development revealed that none of the genes tested proved to be suitable as an internal control. Copy number quantification of the four transcripts showed an average relative variation of seven fold. We propose that the combination of a precise method for RNA quantification, internal controls for monitoring RT reaction and PCR efficiency and a robust external standard curve can guarantee a reliable absolute quantification of mRNA transcripts in real time RT-PCR. This approach may avoid the controversy in the use of housekeeping genes and may assume special significance in tissues undergoing developmental changes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.