ArticleInternational journal of impotence research
Lysophosphatidylcholine, a component of atherogenic lipoproteins, induces the change of calcium mobilization via TRPC ion channels in cultured human corporal smooth muscle cells.
Article in International journal of impotence research. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 33 citations in OpenAlex.
- Targeting TRPC-5 Channel Inhibition to Improve Penile Vascular Function in Erectile Dysfunction.International journal of molecular sciences · 2025Article
- Phospholipases and Reactive Oxygen Species Derived Lipid Biomarkers in Healthy and Diseased Humans and Animals - A Focus on Lysophosphatidylcholine.Frontiers in physiology · 2021Review
- Review
- Potential therapeutic value of transient receptor potential channels in male urogenital system.Pflugers Archiv : European journal of physiology · 2018Review
- The regulation of transient receptor potential canonical 4 (TRPC4) channel by phosphodiesterase 5 inhibitor via the cyclic guanosine 3'5'-monophosphate.Pflugers Archiv : European journal of physiology · 2017Article
- TRPC1/TRPC3 channels mediate lysophosphatidylcholine-induced apoptosis in cultured human coronary artery smooth muscles cells.Oncotarget · 2016Article
- Comprehensive RNA-Seq expression analysis of sensory ganglia with a focus on ion channels and GPCRs in Trigeminal ganglia.PloS one · 2013Article
- Integration of transient receptor potential canonical channels with lipids.Acta physiologica (Oxford, England) · 2012Review
- TRPC channel lipid specificity and mechanisms of lipid regulation.Cell calcium · 2009Review
- Elucidation of a TRPC6-TRPC5 channel cascade that restricts endothelial cell movement.Molecular biology of the cell · 2008Article
- Regulation of eosinophil adhesion by lysophosphatidylcholine via a non-store-operated Ca2+ channel.American journal of respiratory cell and molecular biology · 2007Article
- Cell membrane-derived lysophosphatidylcholine activates cardiac ryanodine receptor channels.Pflugers Archiv : European journal of physiology · 2007Article
- Muscarinic receptor-activated cationic channels in murine ileal myocytes.British journal of pharmacology · 2006Article
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4 authors at 2 institutions in 1 country.
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Abstract
Hypercholesterolemia is a major risk factor for erectile dysfunction. To understand the mechanism(s) of hypercholesterolemia-induced erectile dysfunction, we studied the effect of lysophosphatidylcholine (LPC) on the membrane conductance of corporal smooth muscle cells. We used cultured human corporal smooth muscle cells. The intracelluar Ca2+ concentration ([Ca2+]i) and the influx of divalent cation was monitored by the ratio of fura-2 fluorescence (F(340/380)) and by the Mn2+-induced quenching rate of fura-2, respectively. The LPC-induced membrane current was characterized by the whole-cell patch-clamp technique and the molecular identity of suspected channels was probed by RT-PCR. LPC (20 microM) induced a statistically significant increase in F(340/380) to 119.9+/-3.9% of initial control (n=6) in corporal smooth muscle cells. The addition of 20 microM LPC accelerated the quenching rate of F360 by 59.5+/-11.8% (n=5). LPC activated nonselective cationic current (ILPC), similar to the known effects of phenylephrine in corporal myocytes. The size of ILPC at -60 mV was -55.3+/-6.3 pA (n=8). The transcript of transient receptor potential channel 6 (TRPC6) was detected in human corporal myocytes. We also found one splicing variant of TRPC6, TRPC6alpha. In conclusion, the present study suggests that the LPC, a major component of oxidized low-density lipoprotiens, increases calcium in corporal smooth muscle cells probably through activation of a TRPC6 channel and the increased [Ca2+]i by LPC via TRP channels is one of mechanisms for hypercholesterolemia-induced erectile dysfunction.
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