ArticleNucleic acids research2005
Uracil content of 16S rRNA of thermophilic and psychrophilic prokaryotes correlates inversely with their optimal growth temperatures.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Comparative genomics of crucian carp-isolatedFrontiers in microbiology · 2026Article
- Pseudouridine increases ribosome stability in a thermophilic eukaryote.Nucleic acids research · 2025Article
- Genome-wide identification, characterization and expression analysis of CNGC Genes in moso bamboo (Phyllostachys edulis).BMC plant biology · 2025Article
- Impacts of Groundwater Pumping on Subterranean Microbial Communities in a Deep Aquifer Associated with an Accretionary Prism.Microorganisms · 2024Article
- Genomic basis of environmental adaptation in the widespread poly-extremophilic Exiguobacterium group.The ISME journal · 2024Article
- Identification and expression analysis ofPlant signaling & behavior · 2023Article
- Ribosomal proteins can hold a more accurate record of bacterial thermal adaptation compared to rRNA.Nucleic acids research · 2023Article
- Identification of a deep-branching thermophilic clade sheds light on early bacterial evolution.Nature communications · 2023Article
- The origin and evolution of methanogenesis andPNAS nexus · 2023Article
- Article
- High-resolution structures of a thermophilic eukaryotic 80S ribosome reveal atomistic details of translocation.Nature communications · 2022Article
- Revisiting the Relationships Between Genomic G + C Content, RNA Secondary Structures, and Optimal Growth Temperature.Journal of molecular evolution · 2021Article
- Article
- Extremophile - An Adaptive Strategy for Extreme Conditions and Applications.Current genomics · 2020Review
- TEMPURA: Database of Growth TEMPeratures of Usual and RAre Prokaryotes.Microbes and environments · 2020Article
- Vestiges of Adaptation to the Mesophilic Environment in the Genome of Tepiditoga spiralis gen. nov., sp. nov.Microbes and environments · 2020Article
- Predicting the optimal growth temperatures of prokaryotes using only genome derived features.Bioinformatics (Oxford, England) · 2019Article
- Temperature-dependent expression of different guanine-plus-cytosine content 16S rRNA genes in Haloarcula strains of the class Halobacteria.Antonie van Leeuwenhoek · 2019Article
- Expression and Function of Different Guanine-Plus-Cytosine Content 16S rRNA Genes inFrontiers in microbiology · 2017Article
- Microbial diversity in a submarine carbonate edifice from the serpentinizing hydrothermal system of the Prony Bay (New Caledonia) over a 6-year period.Frontiers in microbiology · 2015Article
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3 authors.
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Abstract
We report here the finding of a highly significant inverse correlation of the uracil content of 16S rRNA and the optimum growth temperature (T(opt)) of cultured thermophilic and psychrophilic prokaryotes. This correlation was significantly different from the weaker correlations between the contents of other nucleotides and T(opt). Analysis of the 16S rRNA secondary structure regions revealed a fall in the A:U base-pair content in step with the increase in T(opt) that was much steeper than that of mismatched base-pairs, which are thermodynamically less stable. These findings indicate that the 16S rRNA sequences of thermophiles and psychrophiles are under a strong thermo-adaptive pressure, and that structure-function constraints play a crucial role in determining their 16S rRNA nucleotide composition. The derived relationship between uracil content and T(opt) was used to develop an algorithm to predict the T(opt) values of uncultured prokaryotes lacking cultured close relatives and belonging to the phyla predominantly containing thermophiles. This algorithm may be useful in guiding the design of cultivation conditions for hitherto uncultured microbes.
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