Evidence map›Paper›PMID 16030322›Full record

ArticleJournal of biomolecular techniques : JBT2005

Analysis of single nucleotide polymorphisms in the promoter region of interleukin-10 by denaturing high-performance liquid chromatography.

Dorothy Guzowski, Alamelu Chandrasekaran, Craig Gawel, Jacqueline Palma, Jonathan Koenig, Xue Ping Wang, Michael Dosik, Mark Kaplan, Charles C Chu, Sangeeta Chavan and 4 more

Abstract readComparative Study
PubMed Full text at PMC
In one paragraph

Article in Journal of biomolecular techniques : JBT, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 13 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 13 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 13 syntheses or guidelines pooled it.

  1. Association between interleukin-10 gene polymorphisms and multiple sclerosis susceptibility: evidence from a meta-analysis.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2026
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  12. Associations between interleukin-10 polymorphisms and susceptibility to psoriasis: a meta-analysis.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2012
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  15. Anti-IL-10 antibody in systemic lupus erythematosus.Open access rheumatology : research and reviews · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Dorothy GuzowskiNorth Shore-LIJ Research Institute, 350 Community Drive, Manhasset, New York 11030, USA. dguzowsk@nshs.edu
Alamelu Chandrasekaran
Craig Gawel
Jacqueline Palma
Jonathan Koenig
Xue Ping Wang
Michael Dosik
Mark Kaplan
Charles C Chu
Sangeeta Chavan
Richard Furie
Emilia Albesiano
Nicholas Chiorazzi
Leslie Goodwin

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Interleukin-10 (IL10), an anti-inflammatory cytokine, has been implicated in a variety of immune- and inflammatory-related diseases. We investigated the following SNPs: -1082, -819, -592 in the promoter region of IL10 in a normal (control) population and selected diseases: breast cancer (BrCa), systemic lupus erythematosus (SLE), and B-cell chronic lymphocytic leukemia (B-CLL) by denaturing high-performance liquid chromatography (DHPLC) and found distinct genotype and haplotype patterns. DHPLC was performed using the Transgenomic WAVE instrument, a mutational discovery tool that allows for high throughout analysis of SNPs. The principle of DHPLC is based on separation of homo- and heteroduplex formation of individual polymerase chain reaction products at specific melting temperatures and set gradients. The melting temperature selected for each SNP was based on size and sequence of the polymerase chain reaction product (for -1082, 57 degrees C; for -819, 58 degrees C; and for -592, 59.2 degrees C). Before fragment mutational analysis, all samples were denatured at 95 degrees C and slowly reannealed to allow for reassociation of different strands. Heteroduplex samples were easily distinguished from homoduplex samples. In order to identify wild type from homozygous mutant, two homoduplex polymerase chain reaction samples had to be mixed together, denatured at 95 degrees C and reannealed. The homozygous mutant, when combined with wild type, displayed a double peak on chromatogram. Once distinct chromatograms were established for each of the SNPs and the nucleotide changes confirmed by sequencing, genotype and haplotype frequencies were tabulated for the groups studied.

Indexed as

Chromatography, High Pressure LiquidPolymorphism, Single NucleotidePromoter Regions, GeneticBase SequenceBreast NeoplasmsFemaleGenetic Predisposition to DiseaseHumansInterleukin-10Leukemia, Lymphocytic, Chronic, B-CellLupus Erythematosus, SystemicMolecular Sequence DataInterleukin-10

Identifiers

PMID16030322
PMCPMC2291722

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.