ArticleMolecular biology of the cell2005
Galpha subunit Gpa2 recruits kelch repeat subunits that inhibit receptor-G protein coupling during cAMP-induced dimorphic transitions in Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 73 citations in OpenAlex.
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- Role of Two G-Protein α Subunits in Vegetative Growth, Cell Wall Integrity, and Virulence of the Entomopathogenic FungusJournal of fungi (Basel, Switzerland) · 2022Article
- The Rpd3-Complex Regulates Expression of Multiple Cell Surface Recycling Factors in Yeast.International journal of molecular sciences · 2021Article
- Diversity ofPersoonia · 2021Article
- G-Protein Subunit GαFrontiers in microbiology · 2020Article
- Phosphorylation of the Gα protein Gpa2 promotes protein kinase A signaling in yeast.The Journal of biological chemistry · 2019Article
- Aggregate Filamentous Growth Responses in Yeast.mSphere · 2019Article
- Fungal G-protein-coupled receptors: mediators of pathogenesis and targets for disease control.Nature microbiology · 2018Review
- Coordinated regulation of intracellular pH by two glucose-sensing pathways in yeast.The Journal of biological chemistry · 2018Article
- KRH1 and KRH2 are functionally non-redundant in signaling for pseudohyphal differentiation in Saccharomyces cerevisiae.Current genetics · 2017Article
- A Ras GTPase associated protein is involved in the phototropic and circadian photobiology responses in fungi.Scientific reports · 2017Article
- Progress toward Understanding Protein S-acylation: Prospective in Plants.Frontiers in plant science · 2017Review
- Phs1 and the synthesis of very long chain Fatty acids are required for ballistospore formation.PloS one · 2014Article
- The role of the RACK1 ortholog Cpc2p in modulating pheromone-induced cell cycle arrest in fission yeast.PloS one · 2013Article
- Analysis of substrate specificity of human DHHC protein acyltransferases using a yeast expression system.Molecular biology of the cell · 2012Article
- Nutritional control of growth and development in yeast.Genetics · 2012Review
- Article
- Two major inositol transporters and their role in cryptococcal virulence.Eukaryotic cell · 2011Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
All eukaryotic cells sense extracellular stimuli and activate intracellular signaling cascades via G protein-coupled receptors (GPCR) and associated heterotrimeric G proteins. The Saccharomyces cerevisiae GPCR Gpr1 and associated Galpha subunit Gpa2 sense extracellular carbon sources (including glucose) to govern filamentous growth. In contrast to conventional Galpha subunits, Gpa2 forms an atypical G protein complex with the kelch repeat Gbeta mimic proteins Gpb1 and Gpb2. Gpb1/2 negatively regulate cAMP signaling by inhibiting Gpa2 and an as yet unidentified target. Here we show that Gpa2 requires lipid modifications of its N-terminus for membrane localization but association with the Gpr1 receptor or Gpb1/2 subunits is dispensable for membrane targeting. Instead, Gpa2 promotes membrane localization of its associated Gbeta mimic subunit Gpb2. We also show that the Gpa2 N-terminus binds both to Gpb2 and to the C-terminal tail of the Gpr1 receptor and that Gpb1/2 binding interferes with Gpr1 receptor coupling to Gpa2. Our studies invoke novel mechanisms involving GPCR-G protein modules that may be conserved in multicellular eukaryotes.
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