ArticleJournal of bacteriology2005
Phenotypic alteration and target gene identification using combinatorial libraries of zinc finger proteins in prokaryotic cells.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 51 citations in OpenAlex.
- Reprogramming cell fate with artificial transcription factors.FEBS letters · 2018Review
- Enhancement of Arabidopsis growth characteristics using genome interrogation with artificial transcription factors.PloS one · 2017Article
- Genome editing comes of age.Nature protocols · 2016Review
- Rapid prototyping of microbial cell factories via genome-scale engineering.Biotechnology advances · 2015Review
- Bacterial sigma factors as targets for engineered or synthetic transcriptional control.Frontiers in bioengineering and biotechnology · 2014Review
- Stabilized homoserine o-succinyltransferases (MetA) or L-methionine partially recovers the growth defect in Escherichia coli lacking ATP-dependent proteases or the DnaK chaperone.BMC microbiology · 2013Article
- Improving acetate tolerance of Escherichia coli by rewiring its global regulator cAMP receptor protein (CRP).PloS one · 2013Article
- Engineering genes for predictable protein expression.Protein expression and purification · 2012Review
- Combinatorial approaches for inverse metabolic engineering applications.Computational and structural biotechnology journal · 2012Review
- Strain engineering for improved expression of recombinant proteins in bacteria.Microbial cell factories · 2011Review
- A multi-functional synthetic gene network: a frequency multiplier, oscillator and switch.PloS one · 2011Article
- Targeted genome engineering via zinc finger nucleases.Plant biotechnology reports · 2011Article
- Induction of stable drug resistance in human breast cancer cells using a combinatorial zinc finger transcription factor library.PloS one · 2011Article
- Modular system for the construction of zinc-finger libraries and proteins.Nature protocols · 2010Article
- Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly.Genome research · 2009Article
- Phenotypic engineering by reprogramming gene transcription using novel artificial transcription factors in Escherichia coli.Nucleic acids research · 2008Article
- Assessing the potential of mutational strategies to elicit new phenotypes in industrial strains.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have developed a method with prokaryotic organisms that uses randomized libraries of zinc finger-containing artificial transcription factors to induce phenotypic variations and to identify genes involved in the generation of a specific phenotype of interest. Combining chromatin immunoprecipitation experiments and in silico prediction of target DNA binding sequences for the artificial transcription factors, we identified ubiX, whose down-regulation correlates with the thermotolerance phenotype in Escherichia coli. Our results show that randomized libraries of artificial transcription factors are powerful tools for functional genomic studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.