ArticleJournal of bacteriology2005
The Holin protein of bacteriophage PRD1 forms a pore for small-molecule and endolysin translocation.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Evaluating phage lytic activity: from plaque assays to single-cell technologies.Frontiers in microbiology · 2025Review
- Characterization of the LysP2110-HolP2110 Lysis System inInternational journal of molecular sciences · 2023Article
- Classification and Multifaceted Potential of Secondary Metabolites Produced byMolecules (Basel, Switzerland) · 2023Review
- Characterizations of novel broad-spectrum lytic bacteriophagesFrontiers in microbiology · 2023Article
- Isolation and Characterization of a NovelViruses · 2022Article
- Indirect Selection against Antibiotic Resistance via Specialized Plasmid-Dependent Bacteriophages.Microorganisms · 2021Article
- Phage S144, A New Polyvalent Phage InfectingInternational journal of molecular sciences · 2020Article
- Half a Century of Research on Membrane-Containing Bacteriophages: Bringing New Concepts to Modern Virology.Viruses · 2019Review
- Characterization and Genome Analysis ofViruses · 2019Article
- Genomic and Proteomic Characterizations ofFrontiers in microbiology · 2019Article
- Genome analysis reveals insights of the endophytic Bacillus toyonensis BAC3151 as a potentially novel agent for biocontrol of plant pathogens.World journal of microbiology & biotechnology · 2017Article
- Identification and classification of known and putative antimicrobial compounds produced by a wide variety of Bacillales species.BMC genomics · 2016Article
- Isolation and Genome Characterization of the Virulent Staphylococcus aureus Bacteriophage SA97.Viruses · 2015Article
- Isolation and Genomic Characterization of the T4-Like Bacteriophage PM2 Infecting Pectobacterium carotovorum subsp. carotovorum.The plant pathology journal · 2015Article
- Characterization and determination of holin protein of Streptococcus suis bacteriophage SMP in heterologous host.Virology journal · 2012Article
- Characterization of LysB4, an endolysin from the Bacillus cereus-infecting bacteriophage B4.BMC microbiology · 2012Article
- Characterization of peptidoglycan hydrolase in Cag pathogenicity island of Helicobacter pylori.Molecular biology reports · 2011Article
- Characterization of a novel LysM domain from Lactobacillus fermentum bacteriophage endolysin and its use as an anchor to display heterologous proteins on the surfaces of lactic acid bacteria.Applied and environmental microbiology · 2010Article
- Particle assembly and ultrastructural features associated with replication of the lytic archaeal virus sulfolobus turreted icosahedral virus.Journal of virology · 2009Article
- Global changes in cellular gene expression during bacteriophage PRD1 infection.Journal of virology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PRD1 is a bacteriophage with an icosahedral outer protein layer surrounding the viral membrane, which encloses the linear double-stranded DNA genome. PRD1 infects gram-negative cells harboring a conjugative IncP plasmid. Here we studied the lytic functions of PRD1. Using infected cells and plasmid-borne lysis genes, we demonstrated that a two-component lysis system (holin-endolysin) operates to release progeny phage particles from the host cell. Monitoring of ion fluxes and the ATP content of the infected cells allowed us to build a model of the sequence of lysis-related physiological changes. A decrease in the intracellular level of ATP is the earliest indicator of cell lysis, followed by the leakage of K+ from the cytosol approximately 20 min prior to the decrease in culture turbidity. However, the K+ efflux does not immediately lead to the depolarization of the cytoplasmic membrane or leakage of the intracellular ATP. These effects are observed only approximately 5 to 10 min prior to cell lysis. Similar results were obtained using cells expressing the holin and endolysin genes from plasmids.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.