ArticleJournal of bacteriology2005
Identification of a D-glycero-D-manno-heptosyltransferase gene from Helicobacter pylori.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Advances in Helicobacter pylori lipopolysaccharide structure and function.FEMS microbiology reviews · 2025Review
- Roles of Lipopolysaccharide Glycosyltransferases in Maintenance ofInternational journal of molecular sciences · 2023Article
- Small RNA mediated gradual control of lipopolysaccharide biosynthesis affects antibiotic resistance in Helicobacter pylori.Nature communications · 2021Article
- Metabolic Glycan Labeling-Based Screen to Identify Bacterial Glycosylation Genes.ACS infectious diseases · 2020Article
- East-Asian Helicobacter pylori strains synthesize heptan-deficient lipopolysaccharide.PLoS genetics · 2019Article
- Review
- Article
- Helicobacter pylori modulates host cell responses by CagT4SS-dependent translocation of an intermediate metabolite of LPS inner core heptose biosynthesis.PLoS pathogens · 2017Article
- The redefinition of Helicobacter pylori lipopolysaccharide O-antigen and core-oligosaccharide domains.PLoS pathogens · 2017Article
- Expression and crystallographic studies of D-glycero-β-D-manno-heptose-1-phosphate adenylyltransferase from Burkholderia pseudomallei.Acta crystallographica. Section F, Structural biology communications · 2017Article
- Helicobacter pylori: bacterial strategy for incipient stage and persistent colonization in human gastric niches.Yonsei medical journal · 2014Article
- The potential of dextran-based glycoconjugates for development of Helicobacter pylori vaccine.Glycoconjugate journal · 2014Article
- Article
- Microwave-assisted sample preparation for rapid and sensitive analysis of H. pylori lipid A applicable to a single colony.Journal of lipid research · 2009Article
- Antimicrobial susceptibility of Canadian isolates of Helicobacter pylori in Northeastern Ontario.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicaleArticle
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have identified a Helicobacter pylori d-glycero-d-manno-heptosyltransferase gene, HP0479, which is involved in the biosynthesis of the outer core region of H. pylori lipopolysaccharide (LPS). Insertional inactivation of HP0479 resulted in formation of a truncated LPS molecule lacking an alpha-1,6-glucan-, dd-heptose-containing outer core region and O-chain polysaccharide. Detailed structural analysis of purified LPS from HP0479 mutants of strains SS1, 26695, O:3, and PJ1 by a combination of chemical and mass spectrometric methods showed that HP0479 likely encodes alpha-1,2-d-glycero-d-manno-heptosyltransferase, which adds a d-glycero-d-manno-heptose residue (DDHepII) to a distal dd-heptose of the core oligosaccharide backbone of H. pylori LPS. When the wild-type HP0479 gene was reintegrated into the chromosome of strain 26695 by using an "antibiotic cassette swapping" method, the complete LPS structure was restored. Introduction of the HP0479 mutation into the H. pylori mouse-colonizing Sydney (SS1) strain and the clinical isolate PJ1, which expresses dd-heptoglycan, resulted in the loss of colonization in a mouse model. This indicates that H. pylori expressing a deeply truncated LPS is unable to successfully colonize the murine stomach and provides evidence for a critical role of the outer core region of H. pylori LPS in colonization.
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