ArticleJournal of bacteriology2005
Carboxyl-terminal extensions beyond the conserved pentapeptide reduce rates of chemoreceptor adaptational modification.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- A chemoreceptor conformational equilibrium controlled by signaling inputs.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Enhanced chemotaxis and degradation of nonylphenol in Pseudoxanthomonas mexicana via CRISPR-mediated receptor modification.Scientific reports · 2025Article
- A Selective Tether Recruits Activated Response Regulator CheB to Its Chemoreceptor Substrate.mBio · 2021Article
- Methyltransferase CheR binds to its chemoreceptor substrates independent of their signaling conformation yet modifies them differentially.Protein science : a publication of the Protein Society · 2020Article
- Flexible Hinges in Bacterial Chemoreceptors.Journal of bacteriology · 2018Article
- Signaling complexes control the chemotaxis kinase by altering its apparent rate constant of autophosphorylation.Protein science : a publication of the Protein Society · 2017Article
- Bacterial Chemoreceptor Dynamics: Helical Stability in the Cytoplasmic Domain Varies with Functional Segment and Adaptational Modification.Journal of molecular biology · 2016Article
- Differential backbone dynamics of companion helices in the extended helical coiled-coil domain of a bacterial chemoreceptor.Protein science : a publication of the Protein Society · 2015Article
- Selective allosteric coupling in core chemotaxis signaling complexes.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Influence of membrane lipid composition on a transmembrane bacterial chemoreceptor.The Journal of biological chemistry · 2012Article
- Direct evidence that the carboxyl-terminal sequence of a bacterial chemoreceptor is an unstructured linker and enzyme tether.Protein science : a publication of the Protein Society · 2011Article
- Structural insight into the low affinity between Thermotoga maritima CheA and CheB compared to their Escherichia coli/Salmonella typhimurium counterparts.International journal of biological macromolecules · 2011Article
- Mutational analysis of the transmembrane helix 2-HAMP domain connection in the Escherichia coli aspartate chemoreceptor tar.Journal of bacteriology · 2011Article
- Chemoreceptors in signalling complexes: shifted conformation and asymmetric coupling.Molecular microbiology · 2010Article
- Chemotactic response and adaptation dynamics in Escherichia coli.PLoS computational biology · 2010Article
- The chemoreceptor dimer is the unit of conformational coupling and transmembrane signaling.Journal of bacteriology · 2010Article
- Comparative genomic and protein sequence analyses of a complex system controlling bacterial chemotaxis.Methods in enzymology · 2007Review
- Precise adaptation in bacterial chemotaxis through "assistance neighborhoods".Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Nanodiscs separate chemoreceptor oligomeric states and reveal their signaling properties.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Similarities and differences in interactions of the activity-enhancing chemoreceptor pentapeptide with the two enzymes of adaptational modification.Journal of bacteriology · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Sensory adaptation in bacterial chemotaxis is mediated by covalent modification of chemoreceptors. Specific glutamyl residues are methylated and demethylated in reactions catalyzed by methyltransferase CheR and methylesterase CheB. In the well-characterized chemosensory systems of Escherichia coli and Salmonella spp., efficient modification by either enzyme is dependent on a conserved pentapeptide sequence, NWETF or NWESF, present at the extreme carboxyl terminus of high-abundance chemoreceptors. To what extent is position at the extreme carboxyl terminus important for pentapeptide-mediated enhancement of adaptational modification? Is this position equally important for enhancement of both enzyme activities? To address these questions, we created forms of high-abundance receptor Tsr or Tar carrying one, six, or eight additional amino acids extending beyond the pentapeptide at their carboxyl termini and assayed methylation, demethylation, deamidation, and ability to mediate chemotaxis. In vitro and in vivo, all three carboxyl-terminal extensions reduced pentapeptide-mediated enhancement of rates of adaptational modification. CheB-catalyzed reactions were more affected than CheR-catalyzed reactions. Effects were less severe for the complete sensory system in vivo than for the minimal system of receptor and modification enzymes in vitro. Notably, extended receptors mediated chemotaxis as efficiently as wild-type receptors, providing a striking example of robustness in chemotactic systems. This could reflect compensatory reductions of rates for both modification reactions, mitigation of effects of slower reactions by the intertwined circuitry of signaling and adaptation, or tolerance of a range of reactions rates for adaptational modification. No matter what the mechanism, the observations provide a challenging test for mathematical models of chemotaxis.
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